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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 9921 - 9940 of 11940 results

  4. pDIRECT_25G

    Plasmid
    #91144
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91144-plasmid-map-sequence-id-182272

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  5. pDIRECT_25I

    Plasmid
    #91146
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91146-plasmid-map-sequence-id-182476

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  8. pSLiP-G2-RacE

    Plasmid
    #188966
    Purpose
    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdown
    Depositor
    Seung-Goo Lee
    Article
    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131
    Inserts
    dCas9
    sgRNA: gttagacgctgattacatggactagg
    racE
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PrhaBAD
    Available Since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    188966-plasmid-map-sequence-id-371637

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  16. pMOD_B2121

    Plasmid
    #91065
    Purpose
    Module B, Promoter: GmUbi, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Promoter
    GmUbi
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91065-plasmid-map-sequence-id-181862

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  18. pMOD_C2516

    Plasmid
    #91084
    Purpose
    Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Promoter
    At7SL
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91084-plasmid-map-sequence-id-182048

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  20. pDIRECT_22D

    Plasmid
    #91136
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91136-plasmid-map-sequence-id-182475

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Showing: 9921 - 9940 of 11940 results