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AP553‐1

Plasmid

#87240

Purpose

gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)

Depositor

Geraldine Seydoux

Article

Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

Insert

gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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UAS-Xbp1spliced

Plasmid

#80628

Purpose

expression of Xbp1 RB isoform in Drosophila

Depositor

Pedro Domingos, Herman Steller

Article

Ryoo et al EMBO J. 2007 Jan 10;26(1):242-52. Epub 2

Insert

Xbp1 (Xbp1 Fly)

Expression

Insect

Mutation

RB isoform

Promoter

hsp70

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Scmh1-3'UTR

Plasmid

#61785

Purpose

Luciferase reporter containing the 3'UTR of mouse Scmh1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Scmh1 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Crtap-3'UTR

Plasmid

#61772

Purpose

Luciferase reporter containing the 3'UTR of mouse Crtap

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Crtap 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pNH (GB0064)

Plasmid

#68165

Purpose

Provides the S. Lycopersicum fruit promoter NH as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlNH promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPUbc (GB0145)

Plasmid

#68170

Purpose

Provides the S. Lycopersicum Ubiquitin carrier protein promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlUbc promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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p2x35S (GB0222)

Plasmid

#68173

Purpose

Provides the CaMV dual 35S Promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

2x35S promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only
DVK_FG

Plasmid

#66070

Purpose

MoClo Destination Vector: (based on pSB1K3) [F:LacZa:G]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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DVK_GH

Plasmid

#66071

Purpose

MoClo Destination Vector: (based on pSB1K3) [G:LacZa:H]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB3_omega1R

Plasmid

#68240

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pT2A11 (GB0143)

Plasmid

#68189

Purpose

Provides the S. Lycopersicum 2A11 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Sl2A11 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pTE8 (GB0144)

Plasmid

#68190

Purpose

Provides the S. Lycopersicum E8 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlE8 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLexAOp (GB0118)

Plasmid

#68181

Purpose

Provides the 4xLexA Operator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

4xOpLexA

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDGB3_omega2R

Plasmid

#68241

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pUNC2001

Plasmid

#67280

Purpose

Expresses MBP-Vibrio cholera photolyase in E. coli, vector pMalcw

Depositor

Aziz Sancar

Article

Worthington et al J Biol Chem. 2003 Oct 3;278(40):39143-54

Insert

Vibrio cholera photolyase

Tags

maltose binding protein

Expression

Bacterial

Mutation

none known

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
pET15b+PCNA3 T180C

Plasmid

#66181

Purpose

Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Mutation

T180C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA3 R112C

Plasmid

#66180

Purpose

Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Mutation

R112C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 E105C

Plasmid

#66176

Purpose

Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Mutation

E105C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pBGSA FPR1 Q258A

Plasmid

#66724

Purpose

Constitutive expression of FPR1 Q258A in mammalian cells.

Depositor

Heini Miettinen

Article

Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

Insert

FPR1 (haplotype 3) (FPR1 Human)

Expression

Mammalian

Mutation

Q258A

Promoter

SR-alpha

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_WTnoGirdles

Plasmid

#62653

Purpose

Wild-type OmpA with aromatic girdle residues replaced by leucine

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_noGirdles

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Aromatic girdle residues mutated to Leu (W7, W15,…

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only