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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,008 results for: T7

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Showing: 9921 - 9940 of 11008 results

  4. pET24TEV-tSHIP2

    Plasmid
    #183771
    Purpose
    Expresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backbone
    Depositor
    William Kerr
    Article
    Sudan et al Front Immunol. 2022 May 4;13:830961. doi
    Insert
    SHIP2 (INPPL1 Human)
    Tags
    6HIS and Tobacco Etch Virus (TEV) Cleavage site
    Expression
    Bacterial
    Mutation
    includes aa18 to aa985 only
    Promoter
    T7 lac
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183771-plasmid-map-sequence-id-362691

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  10. pStr-KDEL_EPV-CXCL9-mCherry-SBP

    Plasmid
    #170678
    Purpose
    Expresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Inserts
    CXCL9 (CXCL9 Synthetic, Human)
    streptavidin-KDEL
    Tags
    SBP and mCherry
    Expression
    Mammalian
    Mutation
    Changed threonine 23 to glutamate (T23E)
    Promoter
    IRES and T7
    Available Since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170678-plasmid-map-sequence-id-335801

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  12. pRD400

    Plasmid
    #163107
    Purpose
    Expression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_eYFP_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available Since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163107-plasmid-map-sequence-id-321848

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  13. pRD188

    Plasmid
    #163099
    Purpose
    Expression of mEos3.2_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_mEos3.2_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Expression
    Mammalian
    Promoter
    CMV promoter; T7 promoter
    Available Since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163099-plasmid-map-sequence-id-321840

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  18. pSMT3-FBF2 SS/R

    Plasmid
    #166976
    Purpose
    Expresses FBF-2 with SS/R mutations in repeat 5 in E. coli for purification and crystallization studies
    Depositor
    Traci Hall
    Article
    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1
    Insert
    FBF-2 (fbf-2 Nematode)
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575…
    Promoter
    T7
    Available Since
    April 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166976-plasmid-map-sequence-id-331635

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  19. pSMT3-FBF2 AS/Y

    Plasmid
    #166974
    Purpose
    Expresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studies
    Depositor
    Traci Hall
    Article
    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1
    Insert
    FBF-2 (fbf-2 Nematode)
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575…
    Promoter
    T7
    Available Since
    April 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166974-plasmid-map-sequence-id-331632

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  20. pSMT3-FBF2 AQ/Y

    Plasmid
    #166975
    Purpose
    Expresses FBF-2 with AQ/Y mutations in repeat 5 in E. coli for purification and crystallization studies
    Depositor
    Traci Hall
    Article
    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1
    Insert
    FBF-2 (fbf-2 Nematode)
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575…
    Promoter
    T7
    Available Since
    April 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166975-plasmid-map-sequence-id-331630

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Showing: 9921 - 9940 of 11008 results