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pEN_hUmiRc2

Plasmid

#25747

Purpose

Entry vector for cloning miR30-based shRNA driven by human U6 promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Gateway Cloning

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-Ssh1

Plasmid

#163602

Purpose

Expresses Protein phosphatase Slingshot homolog 1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+8CmCitrine

Plasmid

#34950

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

mCitrine

Expression

Mammalian

Promoter

SP6

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
pIND20-KNL1-s24a_s60a-dCas9

Plasmid

#248563

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

KNL1-s24a_s60a-dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

KNL1 S24A, S60A; Cas9 D10A, H840A

Promoter

TRE, miniCMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

Plasmid

#191627

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FRBx2

Plasmid

#193571

Purpose

Expresses mCherry tagged with two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mCherry

Tags

FRB (T2098L)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_ACh3.0

Plasmid

#121922

Purpose

express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0

Depositor

Yulong Li

Article

Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

Has Service

AAV9

Insert

GPCR activation based ACh sensor GRAB-ACh3.0

Use

AAV

Promoter

hSyn

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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mIFP12-Farnesyl-5

Plasmid

#56251

Purpose

Localization: Plasma Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Farnesyl (HRAS Human)

Tags

mIFP12

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-mScarlet-SARS-CoV-2-SL1 alone

Plasmid

#191485

Purpose

Lentiviral expression of SARS-CoV-2 5_ UTR SL1 alone

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

SARS-CoV-2 5' UTR SL1 alone (NEWENTRY Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_EBFP2

Plasmid

#167857

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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