We narrowed to 25,364 results for: ina
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Plasmid#218866PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8f
Tags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationQ315LPromoterhsp70Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
delCMV-mCherry-2x-Rhotekin-GBD
Plasmid#214142PurposeRho activity sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xRhotekin-GBD (Rtkn Mouse)
TagsmCherryExpressionMammalianMutationOnly the GTPase binding domain (GBD) is includedPromoterdelCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP777_SnoopCatcher-p65HSF1-CO
Plasmid#211770PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSnoopCatcher-p65HSF1
Tags2xOLLASExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect p65HSF…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-D594A
Plasmid#131717PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable sinceNov. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-C2m2-SNAP
Plasmid#208791PurposeFor AAV production to express C2m2-SNAP in astrocytesDepositorInsertC2m2-SNAP (Mfge8 Mouse)
UseAAVTagsSNAP-tagExpressionMammalianMutationMutated 24 and 45 lysine to asparagine in C2 doma…PromoterGFAP promoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hOKMSOX17FNV
Plasmid#206382PurposeExpresses four genes (human OCT4, KLF4, c-MYC, SOX17FNV) in mammalian cells, for lentivirus generation.DepositorPublicationAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGK EGFP-PPM1H-WT
Plasmid#200084PurposeMammalian expression of wild-type PPM1HDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPPM1H (PPM1H Human)
TagsEGFPExpressionMammalianPromoterPGKAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-GCN5 core
Plasmid#179551Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tollip M240A,F241A
Plasmid#178051PurposeGFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertToll-Interacting Protein (TOLLIP Human)
TagsGFPExpressionMammalianMutationChanged Methionine 240 to Alanine and changed Phe…PromoterCMVAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG
Plasmid#176059PurposeExpressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.DepositorInsertCoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
Tags3xFLAGExpressionMammalianMutationR124A and K125APromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKCsub-YPet-NES
Plasmid#138207PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPKCsub-YPet-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable sinceSept. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-D594G
Plasmid#131719PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CuO-V5 CASC3 siRes 110-480 WT
Plasmid#158543PurposeFor PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 110-480DepositorInsertCASC3; BTZ; MLN51 (CASC3 Human)
TagsV5ExpressionMammalianMutationAmino acids 110-480, C-terminal deletionPromoterCMVAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF3B tail 391-747
Plasmid#134615PurposeMammalian expression of mouse KIF3B 391-747DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKIF3B (Kif5b Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 390 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027
Plasmid#134611PurposeMammalian expression of mouse KIF5A 378-1027DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKIF5A (Kif5a Mouse)
TagsHaloExpressionMammalianMutationWT with deletion of the 377 N-terminal residues.PromoterChicken Beta actinAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor TagBFP2-3XFlag (cyto) WPRE
Plasmid#129421PurposeMADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter proteinDepositorInsertTagBFP2-3Flag
UseMosaic analysis for dual recombinase-mediated cas…Tags3X Flag epitope tagPromoternone (3X polyA to mitigate episomal expression)Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLC-HA active Nedd8-P2A-Hygro
Plasmid#124307PurposeLentiviral vector for expression of HA tagged active or C-terminal cleaved Nedd8-P2A-Hygro casette from a CMV promoterDepositorInsertNedd8 (NEDD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationFive C-terminal amino acids missing compared to w…PromoterCMVAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean-WW-NES
Plasmid#84629PurposeEncodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCerulean-WW-NES
Tags6xHis, Cerulean, Nuclear export signal (NES), T7 …ExpressionMammalianPromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PA-mCit-BAD
Plasmid#113459PurposeProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBCL2 associated agonist of cell death (BAD Human)
TagsProteinA-mCitrineExpressionMammalianPromoterCMVAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-NES
Plasmid#84635PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAMPKsub-YPet-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianPromoterCMVAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only