Addgene

We narrowed to 8,890 results for: Pol;

Showing: 121 - 150 of 8890 results

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pGEMHE-POLArISact

Plasmid

#164970

Purpose

T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

POLArISact

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

T7

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLE2

Plasmid

#160766

Purpose

Suppress POLE2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLD1_1

Plasmid

#160791

Purpose

Suppress POLD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLD1_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLA1_2

Plasmid

#160790

Purpose

Suppress POLA1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLA1_2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLA1_1

Plasmid

#160789

Purpose

Suppress POLA1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLA1_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97309

Purpose

HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HR donor

Use

AAV and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-II 15

Plasmid

#35177

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains the C-terminal repeats 16-52

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pLKO.1B POLE1_2

Plasmid

#160765

Purpose

Suppress POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1B POLE1_1

Plasmid

#160763

Purpose

Suppress POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-II CC

Plasmid

#35180

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains C-terminal domain repeats (1-22)X2

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-Pol-II DC

Plasmid

#35181

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C-terminal domain repeats 1-22 and 31-52

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pXPR_BRD003-sgPOLR2D

Plasmid

#125769

Purpose

constitutive expression of a guide RNA targeting human POLR2D (CRISPR positive control)

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgPOLR2D (POLR2D Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-POLL

Plasmid

#215141

Purpose

Expresses mCherry-POLL in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

POLL

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-POLD1

Plasmid

#215139

Purpose

Expresses mCherry-POLD1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

POLD1 (POLD1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-POLB

Plasmid

#215138

Purpose

Expresses mCherry-POLB in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

POLB (POLB Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pL0_EF-vapol

Plasmid

#202174

Purpose

Level 0 plasmid containing a terminator sequence from gene 47434 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

vapol terminator

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-vapol

Plasmid

#202118

Purpose

Level 0 plasmid containing the promoter from gene 47434 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

vapol promoter

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pXPR_023d-sgCiPOLR2D

Plasmid

#202445

Purpose

Expression of a CRISPRi guide targeting POLR2D

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

POLR2D gRNA (POLR2D Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2CG NBT:NRGIIIa_R551Q:polyA

Plasmid

#193013

Purpose

Tol2 transgenic construct that will express human Neuregulin1 Type IIIa R551Q patient variant in neurons

Depositor

William Talbot

Article

Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

Inserts

NBT
NRG1 type IIIa R551Q (NRG1 Human)

Use

Transposon transgenesis

Tags

Expression

Mammalian

Mutation

ENST00000287842, NM_013956, c.1652G>A, p.(Arg5…

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-POLB-KO-g1

Plasmid

#176090

Purpose

Cas9 plus POLB gRNA #1; contains a puromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

POLB sgRNA #1 (POLB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N0

Plasmid

#160983

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-pol1-N1

Plasmid

#160988

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N1-2A

Plasmid

#160986

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

W84A/D88A

Promoter

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N2-2A

Plasmid

#160987

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

W84A/D88A

Promoter

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGST-POLA1-N2

Plasmid

#160985

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST-POLA1-N1

Plasmid

#160984

Purpose

Express a fragment of mouse Pola1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

POLA1 (Pola1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST-pol1-N2

Plasmid

#160989

Purpose

Express a fragment of yeast pol1 protein

Depositor

Zhiguo Zhang

Article

Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

Insert

pol1

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_PLK1_POLO-box

Plasmid

#109812

Purpose

Protein expression and purification of PLK1_POLO-box

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PLK1_POLO-box (PLK1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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ELL2mRNA+polyA

Plasmid

#127256

Purpose

For in vitro translation of human ELL2 with 30 nt pA tail

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

shortens 3'UTR to 40 bp adds A30 tail

Promoter

T7

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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POLN delP_pDEST17

Plasmid

#64886

Purpose

bacterial expression of human POLN with a short truncation of the C-terminal proline-rich tail

Depositor

Richard Wood

Article

Takata et al J Biol Chem. 2006 Aug 18;281(33):23445-5

Insert

POLN (POLN Human)

Use

Tags

Flag and His

Expression

Bacterial

Mutation

with a short truncation of the C-terminal proline…

Promoter

T7

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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