We narrowed to 9,669 results for: Coli

1. pOpen-Exonuclease III E. coli

   Plasmid

   #165571

   Purpose

   Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   Exonuclease III E. coli
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pOpen-Endonuclease VIII E. coli

   Plasmid

   #165561

   Purpose

   Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities; The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and uracil glycol. The AP lyase activity cleaves DNA phosphodiester backbone at AP sites via beta and delta-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini.

   Depositor

   Jennifer Molloy, FreeGenes Project, Drew Endy

   Insert

   Endonuclease VIII E. coli
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pXTK056 [Con-Pro_glpT-Ecoli_PartType8]

   Plasmid

   #229293

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_glpT-Ecoli_PartType8

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   glpT Promoter (E coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXTK037 [Con-Pro_glpT-Ecoli_PartType5]

   Plasmid

   #229274

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_glpT-Ecoli_PartType5

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   glpT Promoter (E coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK-E.coli rrnB 3'UTR

   Plasmid

   #123573

   Purpose

   MoChlo kit

   Depositor

   C. Neal Stewart, Scott Lenaghan

   Article

   Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

   Insert

   E.coli rrnB 3'UTR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEXP5-NT/6xHis mCherry F30 2xdBroccoli

   Plasmid

   #169233

   Purpose

   Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.

   Depositor

   Dora Tang

   Article

   Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

   Insert

   6xHis mCherry F30-2xdBroccoli
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Dec. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5

   Plasmid

   #124362

   Purpose

   Overexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)

   Depositor

   Samie Jaffrey

   Article

   Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

   Insert

   Tornado-F30-Broccoli-aptNFkB#5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAV-U6+27-Tornado-F30-Squash-Broccoli

   Plasmid

   #177918

   Purpose

   Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.

   Depositor

   Samie Jaffrey

   Article

   Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

   Insert

   Tornado-F30-Squash-Broccoli
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV-U6+27-F30-Tornado-Acyclovir-Broccoli#4

   Plasmid

   #216822

   Purpose

   Expresses acyclovir-controllable Broccoli aptamer #4 as circular RNA in mammalian cells using the Tornado expression system.

   Depositor

   Samie Jaffrey

   Article

   Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

   Insert

   Tornado-Acyclovir-Broccoli#4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYFJ16-LplA(W37V) (for E. Coli expression)

    Plasmid

    #34838

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LplA W37V (lplA E. Coli)
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Mutated Trp37 to Val

    Promoter

    T5 promoter

    Available Since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-E. coli Initiation Factor 3 (IF3)

    Plasmid

    #31340

    Depositor

    Linda Spremulli

    Article

    Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

    Insert

    E. coli Initiation Factor 3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

12. Psicheck2 COLIA1 full length 3'UTR

    Plasmid

    #26993

    Depositor

    Joan Massague

    Article

    Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

    Insert

    COL1A1 Full length 3' UTR (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

13. E. coli 16S toehold switch sensor

    Plasmid

    #110698

    Purpose

    Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    E. coli 16S toehold switch sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-Split-Broccoli-Toehold-Switch

    Plasmid

    #87316

    Purpose

    Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Anti-TRPML3/Mucolipin-3 [N268/19R]

    Plasmid

    #114477

    Purpose

    Mammalian Expression Plasmid of anti-TRPML3/Mucolipin-3 (Human). Derived from hybridoma N268/19.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-TRPML3/Mucolipin-3 (Homo sapiens) recombinant mouse monoclonal antibody (MCOLN3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    dual CMV

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. aav-U6-broccoli-hsp68-mCherry-MCS-pA

    Plasmid

    #195411

    Purpose

    AAV9 based enhancer activity assay

    Depositor

    William Pu

    Article

    Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-28c-F30-2xdBroccoli-47CAG

    Plasmid

    #237217

    Purpose

    Express F30-2xdBroccoli tagged 47 repeats of CAG RNA in bacteria.

    Depositor

    Mingxu You

    Article

    Xue et al Nucleic Acids Res. 2023 Sep 8;51(16):833

    Insert

    47 CAG trinucleotide repeats RNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEXP5-NT/6xHis mCherry F30 Broccoli

    Plasmid

    #169234

    Purpose

    Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with Broccoli RNA aptamer.

    Depositor

    Dora Tang

    Article

    Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

    Insert

    6xHis mCherry F30-Broccoli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. E. coli mRNA toehold switch sensor

    Plasmid

    #110708

    Purpose

    Toehold switch sensor to detect a species specific mRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    E. coli species specific toehold switch sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

    Plasmid

    #190162

    Purpose

    Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

    Insert

    mcherry_(3'UTR, No Actin)_24Broccoli
    

    Use

    Tags

    24×Broccoli

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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