We narrowed to 9,822 results for: Coli

1. pOpen-Exonuclease III E. coli

   Plasmid

   #165571

   Purpose

   Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   Exonuclease III E. coli
   

   Use

   Synthetic Biology

   Available Since

   Aug. 24, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pOpen-Endonuclease VIII E. coli

   Plasmid

   #165561

   Purpose

   Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities; The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and uracil glycol. The AP lyase activity cleaves DNA phosphodiester backbone at AP sites via beta and delta-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   Endonuclease VIII E. coli
   

   Use

   Synthetic Biology

   Available Since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pXTK056 [Con-Pro_glpT-Ecoli_PartType8]

   Plasmid

   #229293

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_glpT-Ecoli_PartType8

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   glpT Promoter (E coli)
   

   Expression

   Bacterial

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pXTK037 [Con-Pro_glpT-Ecoli_PartType5]

   Plasmid

   #229274

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_glpT-Ecoli_PartType5

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   glpT Promoter (E coli)
   

   Expression

   Bacterial

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMK-E.coli rrnB 3'UTR

   Plasmid

   #123573

   Purpose

   MoChlo kit

   Depositor

   Scott Lenaghan, C. Neal Stewart

   Article

   Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

   Insert

   E.coli rrnB 3'UTR
   

   Use

   Synthetic Biology

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5

   Plasmid

   #124362

   Purpose

   Overexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)

   Depositor

   Samie Jaffrey

   Article

   Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

   Insert

   Tornado-F30-Broccoli-aptNFkB#5
   

   Expression

   Mammalian

   Promoter

   U6+27

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Anti-TRPML3/Mucolipin-3 [N268/19R]

   Plasmid

   #114477

   Purpose

   Mammalian Expression Plasmid of anti-TRPML3/Mucolipin-3 (Human). Derived from hybridoma N268/19.

   Depositor

   James Trimmer

   Article

   Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-TRPML3/Mucolipin-3 (Homo sapiens) recombinant mouse monoclonal antibody (MCOLN3 Mouse)
   

   Expression

   Mammalian

   Promoter

   dual CMV

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAV-U6+27-Tornado-F30-Squash-Broccoli

   Plasmid

   #177918

   Purpose

   Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.

   Depositor

   Samie Jaffrey

   Article

   Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

   Insert

   Tornado-F30-Squash-Broccoli
   

   Expression

   Mammalian

   Promoter

   U6+27

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-28c-F30-2xdBroccoli-47CAG

   Plasmid

   #237217

   Purpose

   Express F30-2xdBroccoli tagged 47 repeats of CAG RNA in bacteria.

   Depositor

   Mingxu You

   Article

   Xue et al Nucleic Acids Res. 2023 Sep 8;51(16):833

   Insert

   47 CAG trinucleotide repeats RNA
   

   Expression

   Bacterial

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEXP5-NT/6xHis mCherry F30 2xdBroccoli

    Plasmid

    #169233

    Purpose

    Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.

    Depositor

    Dora Tang

    Article

    Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

    Insert

    6xHis mCherry F30-2xdBroccoli
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAV-U6+27-F30-Tornado-Acyclovir-Broccoli#4

    Plasmid

    #216822

    Purpose

    Expresses acyclovir-controllable Broccoli aptamer #4 as circular RNA in mammalian cells using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

    Insert

    Tornado-Acyclovir-Broccoli#4
    

    Expression

    Mammalian

    Promoter

    U6+27

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYFJ16-LplA(W37V) (for E. Coli expression)

    Plasmid

    #34838

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LplA W37V (lplA E. Coli)
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Mutated Trp37 to Val

    Promoter

    T5 promoter

    Available Since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21-E. coli Initiation Factor 3 (IF3)

    Plasmid

    #31340

    Depositor

    Linda Spremulli

    Article

    Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

    Insert

    E. coli Initiation Factor 3
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

14. Psicheck2 COLIA1 full length 3'UTR

    Plasmid

    #26993

    Depositor

    Joan Massague

    Article

    Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

    Insert

    COL1A1 Full length 3' UTR (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Available Since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

15. E. coli 16S toehold switch sensor

    Plasmid

    #110698

    Purpose

    Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    E. coli 16S toehold switch sensor
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19-Split-Broccoli-Toehold-Switch

    Plasmid

    #87316

    Purpose

    Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    P70a

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. aav-U6-broccoli-hsp68-mCherry-MCS-pA

    Plasmid

    #195411

    Purpose

    AAV9 based enhancer activity assay

    Depositor

    William Pu

    Article

    Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

    Type

    Empty backbone

    Use

    AAV

    Available Since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEXP5-NT/6xHis mCherry F30 Broccoli

    Plasmid

    #169234

    Purpose

    Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with Broccoli RNA aptamer.

    Depositor

    Dora Tang

    Article

    Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

    Insert

    6xHis mCherry F30-Broccoli
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. E. coli mRNA toehold switch sensor

    Plasmid

    #110708

    Purpose

    Toehold switch sensor to detect a species specific mRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    E. coli species specific toehold switch sensor
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

    Plasmid

    #190162

    Purpose

    Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

    Insert

    mcherry_(3'UTR, No Actin)_24Broccoli
    

    Tags

    24×Broccoli

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge