Addgene

We narrowed to 35,360 results for: ESC;

Showing: 81 - 100 of 35360 results

Results per page:

Sort results by:

pUASt-ssBiP-ESCargo

Plasmid

#140166

Purpose

Expresses a regulatable secretory cargo for Drosophila melanogaster

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Insect

Mutation

Promoter

hs70

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASt-ssBiP-ESCargo*

Plasmid

#140165

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogaster

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssBip-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Insect

Mutation

Promoter

hs70

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Rescue Septin 11 mRNA

Plasmid

#122025

Purpose

Rescue mRNA for Septin 11 Knock Down with sh2 shRNA. Splice variant II (EU711415) with 5 silent mutations at SEPT11 sh2 shRNA target site.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

Expression

Mammalian

Mutation

Silent mutations are in 5 consecutive codons (CUG…

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pESC-LEU2-RAD30cmyc

Plasmid

#60821

Purpose

to overexpress yeast polymerase eta in yeast

Depositor

Youri Pavlov, Thomas Kunkel

Article

Pavlov et al Mutat Res. 2001 Jul 1;478(1-2):129-39.

Insert

RAD30 (RAD30 Budding Yeast)

Use

Tags

c-myc

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pBluescript-KS(+) CREST (C90)

Plasmid

#22962

Depositor

Anirvan Ghosh

Insert

CREST (Ss18l1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only
HOXB5 (human) pBluescript

Plasmid

#8531

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB5 (HOXB5 Human)

Use

Tags

Expression

Bacterial

Mutation

makes protein in vitro transcription/translation

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
human EB1 in pBluescript II

Plasmid

#39324

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pBluescript-KS-Nrg3-ISH

Plasmid

#87669

Purpose

In situ probe for mouse Nrg3

Depositor

Oscar Marin

Article

Bartolini et al Cell Rep. 2017 Jan 31;18(5):1157-1170. d

Insert

Nrg3 (Nrg3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7/M13

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
564aa ELL2 bluescript

Plasmid

#127267

Purpose

For in vitro translation of human ELL2 lacking COOH region

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

Addgene Plasmid 127256--SphI site filled in, then…

Promoter

T7

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ESCO2 C10.2 gRNA

Plasmid

#90681

Purpose

3rd generation lentiviral gRNA plasmid targeting human ESCO2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ESCO2 (Guide Designation C10.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
ESCO2 C9.2 gRNA

Plasmid

#90680

Purpose

3rd generation lentiviral gRNA plasmid targeting human ESCO2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ESCO2 (Guide Designation C9.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript SK-rPAI-153

Plasmid

#45389

Depositor

Thomas Gelehrter

Article

Zeheb et al Gene. 1988 Dec 20;73(2):459-68.

Insert

Plasminogen activator inhibitor type 1 (Serpine1 Rat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
ESCRT-I

Plasmid

#17634

Depositor

James Hurley

Article

Kostelansky et al Cell. 2006 Apr 7. 125(1):113-26.

Insert

Vps23(1-385) – His/Vps28(1-242) – Vps37(1-213) – Mvb12(1-101) (VPS28, STP22, SRN2 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD02_CBASS_Escherichia_coli_SLK172

Plasmid

#224395

Purpose

For expression of the type I CBASS system encoded by Escherichia coli SLK172

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

EcCdnD_4TM

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBluescript-PB

Plasmid

#133885

Purpose

piggyBac ITR's in high copy number pBluescript backbone plasmid

Depositor

Mario Capecchi

Type

Empty backbone

Use

Piggybac

Tags

Expression

Mutation

Promoter

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
doraWT_rescue_construct

Plasmid

#190609

Purpose

Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Actin-5c

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9_sgRNA_expression_in_pBluescript

Plasmid

#122089

Purpose

U6 driven SpCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9 tracr (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
NmCas9_sgRNA_expression_in_pBluescript

Plasmid

#122091

Purpose

U6 driven NmCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

NmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
SaCas9_sgRNA_expression_in_pBluescript

Plasmid

#122090

Purpose

U6 driven SaCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD02_CBASS_Escherichia_coli_CVM_N37067PS

Plasmid

#224403

Purpose

For expression of the type I CBASS system encoded by Escherichia coli CVM N37067PS

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

EcCdnF_AGS-C_SLATT6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge