We narrowed to 11,199 results for: NSI;

1. pC027 - LweCas13a from Listeria weihenstephanensis FSL R9-0317

   Plasmid

   #91917

   Purpose

   Expresses LweCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LweCas13a and guide expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Desmoplakin II donor only control (FL-based tension sensor)

   Plasmid

   #118723

   Purpose

   The donor only (YPet(short)) control for the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin II-[YPet(short)-FL-mCherry(Y72L)] (internal-1353) (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)-FL-mCherry(Y72L)

   Expression

   Mammalian

   Mutation

   inserted FL-based tension sensor module after aa1…

   Promoter

   CMV

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB700 lenti gRNA KanR neo_TtoC and hairpin extension

   Plasmid

   #167925

   Purpose

   Lentiviral vector for expressing U6 FE-sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Desmoplakin I acceptor only control (F40-based tension sensor)

   Plasmid

   #119189

   Purpose

   The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Desmoplakin I donor only control (F40-based tension sensor)

   Plasmid

   #119188

   Purpose

   The donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Desmoplakin II donor only control (F40-based tension sensor)

   Plasmid

   #118717

   Purpose

   The donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin II-[YPet(short)-F40-mCherry(Y72L)] (internal-1353) (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)-F40-mCherry(Y72L)

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   CMV

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Desmoplakin II acceptor only control (F40-based tension sensor)

   Plasmid

   #118719

   Purpose

   The acceptor (mCherry) only control for the F40-based human desmoplakin II tension sensor can be co-expressed with the donor (YPet(short)) only control to determine intermolecular FRET.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin II-[YPet(short)(Y67G)-F40-mCherry] (internal-1353) (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)(Y67G)-F40-mCherry

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   CMV

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CIDAR MoClo Extension, Volume I

   Plasmid Kit

   #1000000161

   Purpose

   This kit contains 110 plasmids designed to quickly and efficiently build a wide variety of gene expression constructs using the CIDAR-standard MoClo system.

   Depositor

   Richard Murray

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

9. EcoFlex MoClo Extension, Volume I

   Plasmid Kit

   #1000000253

   Purpose

   Use the EcoFlex extension kit (volume one) to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system.

   Depositor

   Mikhail Shapiro

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

10. pAM5085

    Plasmid

    #85122

    Purpose

    PkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    PkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
    

    Use

    Tags

    ECFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMT-FLAG-CBPΔN

    Plasmid

    #182840

    Purpose

    A 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2009 Sep;136(18):3131-41. d

    Insert

    CBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
    

    Use

    Tags

    FLAG tag

    Expression

    Insect

    Mutation

    Promoter

    MT

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJMH021

    Plasmid

    #186634

    Purpose

    contains homology arms for the NSI (NIES39_Q01230) locus of Arthrospira platensis

    Depositor

    Adam Arkin

    Article

    Jacob M. et al bioRxiv

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJMH036

    Plasmid

    #186637

    Purpose

    aadA.co between homology arms for NSI

    Depositor

    Adam Arkin

    Article

    Jacob M. et al bioRxiv

    Insert

    aadA.co
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. E. coli K-12 MG1655_rLP5(nth-ydgR)

    Bacterial Strain

    #110245

    Purpose

    E. coli K-12 MG1655 with Landing Pad engineered into the nth-ydgR intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Bacterial Resistance

    Chloramphenicol

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

15. E. coli K-12 MG1655_fLP4(essQ-cspB)

    Bacterial Strain

    #110244

    Purpose

    E. coli K-12 MG1655 with Landing Pad engineered into the essQ-cspB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Bacterial Resistance

    Chloramphenicol

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

16. E. coli K-12 MG1655_rLP6(ygcE-ygcF)

    Bacterial Strain

    #110246

    Purpose

    E. coli K-12 MG1655 with Landing Pad engineered into the ygcE-ygcF intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Bacterial Resistance

    Chloramphenicol

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

17. E. coli K-12 MG1655_rLP1(atpI-gidB)

    Bacterial Strain

    #110241

    Purpose

    E. coli K-12 MG1655 with Landing Pad engineered into the atpl-gidB intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Bacterial Resistance

    Chloramphenicol

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

18. E. coli K-12 MG1655_fLP3(ybbD-ylbG)

    Bacterial Strain

    #110243

    Purpose

    E. coli K-12 MG1655 with Landing Pad engineered into the ybbD-ylbG intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Bacterial Resistance

    Chloramphenicol

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

19. E. coli K-12 MG1655_fLP2(yieN-trkD)

    Bacterial Strain

    #110242

    Purpose

    E. coli K-12 MG1655 with Landing Pad engineered into the yieN-trkD intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Bacterial Resistance

    Chloramphenicol

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

20. B95(DE3) ΔA ΔfabR ΔserB

    Bacterial Strain

    #197655

    Purpose

    This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

    Depositor

    Ryan Mehl, Richard Cooley

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Bacterial Resistance

    None

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only