-
Plasmid#74753PurposeExpressed sfGFP-tagged FIP3 in E. coli for protein productionDepositorInsertRab11 family interacting protein 3 (RAB11FIP3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterT7Available sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET17_K560_GFP_His
Plasmid#15219DepositorInsertHuman neuronal kinesin heavy chain (KIF5B Human)
UseTagsGFP and HisExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
His10-CD3z-SNAPf-ybbR (XSB507)
Plasmid#78508PurposeConstruct for expressing CD3z-SNAPf-ybbR in E. ColiDepositorInsertCD3z (Cytoplasmic domain) (CD247 Human)
UseTagsHis10 and SNAPf-ybbRExpressionBacterialMutationPromoterAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Emi1 5' and 3'UTR
Plasmid#67930PurposeTranslational reporter - Emi1 UTRsDepositorInsertsfGFP-NLS-P2A-NLS-mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ ARHGAP5 5' and 3'UTR
Plasmid#67931PurposeTranslational reporter - ARHGAP5 UTRsDepositorInsertsfGFP-NLS-P2A-NLS-mCherry (ARHGAP5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
PERK 1: PERK.WT.9E10.pCDNA
Plasmid#21814DepositorInsertPERK (Eif2ak3 Mouse)
UseTagsMyc (9E10)ExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-548e-5p
Plasmid#103634PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-548e-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-548e-5p target (MIR548E Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-199a-3p
Plasmid#103319PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-199a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-199a-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
p3xFLAG-MKL1
Plasmid#11978PurposeFor mammalian expression of human MKL1 with a 3x FLAG tagDepositorInsertMKL1 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationN579S T716APromoterCMVAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFDH
Plasmid#131706PurposeExpress constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coliDepositorInsertformate dehydrogenase
UseTagshis-tagExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-mito-mCherry-24xGCN4_v1
Plasmid#60913PurposeA fusion protein of the SunTag 24xGCN4 v1 peptide array to a mitochondrial targeting domain and mCherryDepositorInsertmito-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-H2B-SunTag24x_v1
Plasmid#60911PurposeEnocdes a histone H2B fused to the SunTag for single molecule imagingDepositorInsertH2B
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3XFLAG-MKL2
Plasmid#27175PurposeFor mammalian expression of human MKL2 with a 3x FLAG tagDepositorInsertMKL2 (MRTFB Human)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHR_dSV40-Aurora A-GFP
Plasmid#67924PurposeExpresses Aurora A-GFP in mammalian cellsDepositorInsertGFP-Aurora A (AURKA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTW072M
Plasmid#115960PurposeDestination VectorDepositorInsertLvl2 (nsP2 Q739L)
UseSynthetic BiologyTagsExpressionMutationnsP2 Q739LPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
PERK 5: PERK.K618R.pGEX4T-1
Plasmid#21818DepositorInsertPERK (537-1114) K618R (Eif2ak3 Mouse)
UseTagsGST and Myc (9E10)ExpressionBacterialMutationPERK Kinase Domain. Amino acids 537-1114. Kinase …PromoterAvailable sinceMarch 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHR CD22-Megf10 CAR
Plasmid#113016Purposefor production of lentivirus encoding CD22 CAR-P Megf10DepositorInsertCD22 CAR-P Megf10 intracellular domain
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUT8-Dox
Plasmid#124631PurposeDoxycycline-inducible FUT8 expression in mammalian cellsDepositorInsertsAlpha-(1,6)-fucosyltransferase
Tetracycline repressor A3 mutant
TagFP635
UseTagsExpressionMammalianMutationPromoterTRE-tight and hEF1aAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only