Skip to main content
Addgene
Showing: 81 - 100 of 378 results

  1. pCSJ125

    Plasmid
    #104344
    Purpose
    Express yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
    Depositor
    Alexander Varshavsky
    Article
    Chen et al Science. 2017 Jan 27;355(6323). pii: 355
    Insert
    MDH2
    Use
    Tags
    3xFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104344-plasmid-map-sequence-id-207905

    Enlarge

  2. pJO629

    Plasmid
    #104342
    Purpose
    Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
    Depositor
    Alexander Varshavsky
    Article
    Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2
    Insert
    pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
    Use
    Tags
    3xFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104342-plasmid-map-sequence-id-207903

    Enlarge

  3. pCSJ982

    Plasmid
    #176622
    Purpose
    Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
    Depositor
    Alexander Varshavsky
    Article
    Dong et al Proc Natl Acad Sci U S A. 2020 Jun 23;11
    Insert
    Ub-MPGLW-Fbp1
    Use
    Tags
    Ubiquitin N-terminal fusion and triple flag tag C…
    Expression
    Yeast
    Mutation
    Changed first 4 N-terminal amino acid residues of…
    Promoter
    TDH3tc3
    Available since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    176622-plasmid-map-sequence-id-356527

    Enlarge

  4. p2B-55

    Plasmid
    #177924
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    TDH3
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177924-plasmid-map-sequence-id-349994

    Enlarge

  5. pBV163

    Plasmid
    #108364
    Purpose
    Used to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.
    Depositor
    Scott Moye-Rowley
    Article
    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0
    Insert
    Knock-in Cg TDH3 promoter in Cg YAP1 gene
    Use
    Replace c.glabrata yap1 promoter with c. glabrata…
    Tags
    Expression
    Mutation
    Promoter
    TDH3
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108364-plasmid-map-sequence-id-209913

    Enlarge

  6. pAL005

    Plasmid
    #233865
    Purpose
    MoClo-YTK URA multigene plasmid with MCS; ABA systems; pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2
    Depositor
    Jeff Hasty
    Article
    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102
    Insert
    pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    233865-plasmid-map-sequence-id-468718

    Enlarge

Showing: 81 - 100 of 378 results