Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 81 - 100 of 1291 results

  4. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

    Plasmid
    #40131
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Use
    Tags
    FokI nuclease (KKR)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    40131-plasmid-map-sequence-id-51019

    Enlarge

  5. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

    Plasmid
    #40132
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Use
    Tags
    FokI nuclease (ELD)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    40132-plasmid-map-sequence-id-51020

    Enlarge

  6. RV-Cag-Dio-mVenus

    Plasmid
    #153202
    Purpose
    Can be used to generate retrovirus that will mark the membrane (Palmitoylated-Venus) in the presence of Cre from the CAG promoter
    Depositor
    Lynette Lim
    Insert
    membrane-tagged-Venus
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    153202-plasmid-map-sequence-id-305047

    Enlarge

  8. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40

    Plasmid
    #100054
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to mCherry driven by CAG promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV1, AAV2, AAV5, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R mutation in ChR2
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    100054-plasmid-map-sequence-id-183171

    Enlarge

  9. pLenti.CAG.H2B-cerFP-2A-mito-dsRFP.W

    Plasmid
    #51013
    Purpose
    Lentivector that expresses H2B-cerFP-2A-mito-dsRFP from internal CAG enhancer-promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-cerFP-2A-mito-RFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    51013-plasmid-map-sequence-id-77778

    Enlarge

  11. pCAGGS-PHFF-sh-hBVRA

    Plasmid
    #100285
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Inserts
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for human BVRA
    Use
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter and H1 promoter
    Availability
    Academic Institutions and Nonprofits only
    100285-plasmid-map-sequence-id-194439

    Enlarge

  12. pCAGGS-PHFF-sh-mBVRA

    Plasmid
    #100286
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Inserts
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for mouse BVRA
    Use
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter and H1 promoter
    Availability
    Academic Institutions and Nonprofits only
    100286-plasmid-map-sequence-id-194443

    Enlarge

  20. AAV-CAG-K-SPOTIT

    Plasmid
    #191488
    Purpose
    Green fluorescent-based opioid sensor for the kappa opioid receptor in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-SPOTIT
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    191488-plasmid-map-sequence-id-377866

    Enlarge

Showing: 81 - 100 of 1291 results