We narrowed to 1,980 results for: cas9 expression vector

1. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #112922

   Purpose

   Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

   Plasmid

   #112921

   Purpose

   Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-Cas9-TagBFP

   Plasmid

   #236725

   Purpose

   Cloning vector Cas9-TagBFP

   Depositor

   David Bersten

   Insert

   Cas9-TagBFP
   

   Use

   CRISPR

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-hCas9D10A

   Plasmid

   #52101

   Purpose

   The expression vector for humanized nickase Cas9 (Cas9D10A) under CAG promoter

   Depositor

   Izuho Hatada

   Insert

   hCas9D10A
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG

   Available Since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pDe-CAS9

   Plasmid

   #61433

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   PcUBI4-2

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9-EcRT-Z3-Kan

   Plasmid

   #232096

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Z3

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10

   Plasmid

   #196079

   Purpose

   Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting Adam10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-A10

   Plasmid

   #196081

   Purpose

   Inducible CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting Adam10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-A10

   Plasmid

   #196083

   Purpose

   Inducible CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting Adam10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196075

    Purpose

    Constitutive CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196077

    Purpose

    Constitutive CRISPRi vector conferring hygromycin B resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122090

    Purpose

    U6 driven SaCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDSAY-vasaCas9sv40

    Plasmid

    #173669

    Purpose

    Cas9 expression vector for Anopheles

    Depositor

    Eric Marois

    Insert

    Cas9 under control of vasa promoter and SV40 terminator
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    vasa

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti.Cas9.BFP.sgRNA.parental

    Plasmid

    #196715

    Purpose

    Sortable WT-SpCas9 expression and guide cassette (1-vector system).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD

    Plasmid

    #196086

    Purpose

    Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting lncRNA NORAD
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196085

    Purpose

    Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid

    #214690

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr1
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDual_dsCas9_Blast_hs_TP73

    Plasmid

    #214691

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73

    Depositor

    Kevin Janes

    Insert

    dgRNA_TP73 (TP73 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDual_dsCas9_Venus_hs_NC_chr1

    Plasmid

    #214682

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr1
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDual_dsCas9_Venus_hs_NC_chr2

    Plasmid

    #214683

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr2
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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