We narrowed to 537 results for: gcg.2

1. pX459-sgRNA2-2-RPB1-C-term

   Plasmid

   #195109

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 C-term. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against last exon of RPB1 (C-terminal)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDY2260 hNOLC1 atgRNA Paired Guide 2

   Plasmid

   #220990

   Purpose

   hNOLC1 atgRNA

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   NOLC1 atgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDY2262 hACTB atgRNA Paired Guide 2

   Plasmid

   #220992

   Purpose

   hACTB atgRNA

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   ACTB atgRNA (ACTB Human)
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. RPS4X sgRNA-2 lentiCRISPR v2 plasmid

   Plasmid

   #192232

   Purpose

   lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-2 targeting eIF3 binding site of RPS4X 5'UTR (RPS4X Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(TRIM25_5-2)-PGKpuroBFP-W

   Plasmid

   #211990

   Purpose

   Express gRNA against TRIM25 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting TRIM25 (TRIM25 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(ID1_5-2)-PGKpuroBFP-W

   Plasmid

   #211964

   Purpose

   Express gRNA against ID1 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting ID1 (ID1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-H11r1-2-DD-Cas9

   Plasmid

   #85978

   Purpose

   Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

   Depositor

   Michele Calos

   Article

   Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

   Insert

   DD-SpCas9
   

   Use

   CRISPR

   Tags

   FKBP12 L106P DD

   Expression

   Mammalian

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1 puro shPRDM14-2

   Plasmid

   #193695

   Purpose

   Constitutive lentiviral expression of PRDM14 shRNA

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   PRDM14 (PRDM14 Human)
   

   Use

   Lentiviral

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORANGE GFP-Actb KI #2

   Plasmid

   #139666

   Purpose

   Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-guide-puro mSptlc2 - 2

    Plasmid

    #198494

    Purpose

    lentiviral stable expression of mSptlc2 gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mSptlc2 (Sptlc2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPR v2-sgSLC7A11/xCT-2

    Plasmid

    #161819

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    solute carrier family 7 member 11 (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. 161+80pCalb2/mutCREB(-2)-like

    Plasmid

    #66751

    Purpose

    luciferase reporter for Calb2 promoter (-161to +80, mutant)

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-161bp +80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    GCGC (-2) to TAGA

    Promoter

    Calb2

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPR v2 puro - CRBN (#2)

    Plasmid

    #166241

    Purpose

    sgRNA (#2) to generate a knockout of human CRBN by targeting the start region of Exon1

    Depositor

    Günter Schneider

    Article

    Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

    Insert

    CRBN (CRBN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

    Plasmid

    #128760

    Purpose

    Expresses PARN gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    PARN sgRNA #2 (PARN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRRL-mouse cGAS #2-gRNA-Cas9-Puro

    Plasmid

    #186891

    Purpose

    gRNA targeting mouse cGAS (with Cas9 insert)

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Cas9 (Cgas Mouse)
    

    Use

    Lentiviral

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. RPS15A sgRNA-2 lentiCRISPR v2 plasmid

    Plasmid

    #192228

    Purpose

    lentiviral vector expressing sgRNA-2 targeting eIF3 binding site of RPS15A 5'UTR

    Depositor

    Dieter Wolf

    Article

    Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

    Insert

    sgRNA-2 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-2)

    Plasmid

    #236613

    Purpose

    Knockouts ANKRD11 in human cells

    Depositor

    Tadashi Nakagawa

    Article

    Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

    Insert

    ANKRD11 (ANKRD11 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-FLEX-SaCas9-U6-sgTh(2)

    Plasmid

    #209198

    Purpose

    Mutagenesis of Th

    Depositor

    Larry Zweifel

    Article

    Yu et al Neuron. 2021 Apr 21;109(8):1365-1380.e5.

    Insert

    Th (Th Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Promoter

    CMV

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSUPER-hHSF2 shRNA

    Plasmid

    #118346

    Purpose

    This plasmid expresses shRNA against hHSF2.

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    hHSF2 shRNA (HSF2 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSUPER-scramble

    Plasmid

    #118349

    Purpose

    This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    scrambled shRNA
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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