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Showing: 81 - 100 of 194 results

  2. pBRα

    Plasmid
    #53731
    Purpose
    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    αNTD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53731-plasmid-map-sequence-id-83909

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  6. pBRα-β flap (831-1057)

    Plasmid
    #53734
    Purpose
    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    alpha NTD-Beta flap fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    53734-plasmid-map-sequence-id-83920

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  7. pAJE-Ma pylRS nitroY/haloY-F5

    Plasmid
    #225684
    Purpose
    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.
    Depositor
    Ryan Mehl
    Inserts
    M. alvus PylRS 3-NY/HaloY F5
    M. alvus Pyl-tRNA(6)
    Use
    Methanomethylophilus alvus
    Tags
    Expression
    Mutation
    CTG125CTT, N166A, V168S, A223C, W239R
    Promoter
    GlnS and Lpp
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    225684-plasmid-map-sequence-id-450701

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  9. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid
    #212120
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    Use
    Tags
    His6 and SUMO-TAG35
    Expression
    Bacterial
    Mutation
    E35TAG (within SUMO encoding sequence)
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212120-plasmid-map-sequence-id-444184

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  10. pKB239

    Plasmid
    #170034
    Purpose
    Encodes LacI-controlled expression of surface-displayed GNLY and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Granulysin (GNLY Human, Synthetic, Escherichia coli)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170034-plasmid-map-sequence-id-336785

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  11. pKB234.2

    Plasmid
    #170023
    Purpose
    Encodes LacI-controlled expression of surface-displayed hBD3 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    hBD3 Δ alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Dec. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    170023-plasmid-map-sequence-id-335944

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  12. pKB237.1

    Plasmid
    #170031
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL19 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    CCL19 Δ alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170031-plasmid-map-sequence-id-336779

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  13. pKB235.4

    Plasmid
    #170026
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL20 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    CCL20 Δ alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Availability
    Academic Institutions and Nonprofits only
    170026-plasmid-map-sequence-id-335946

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  14. pKB236.1

    Plasmid
    #170028
    Purpose
    Encodes LacI-controlled expression of surface-displayed TCP fragment lacking C-terminal alpha-helix sequence and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    TCP Δ C-terminal alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Availability
    Academic Institutions and Nonprofits only
    170028-plasmid-map-sequence-id-335958

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  15. pKB236.2

    Plasmid
    #170029
    Purpose
    Encodes LacI-controlled expression of surface-displayed TCP C-terminal alpha-helix fragment and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    TCP C-terminal alpha-helix
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Availability
    Academic Institutions and Nonprofits only
    170029-plasmid-map-sequence-id-335296

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  16. pBRα-β flap (831-1057)

    Plasmid
    #53734
    Purpose
    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    alpha NTD-Beta flap fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    53734-plasmid-map-sequence-id-83920

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  17. pKB237

    Plasmid
    #170030
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL19 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    CCL19 (CCL19 Human, Synthetic, Escherichia coli)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    170030-plasmid-map-sequence-id-335295

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  18. pBRα-σ70 D581G

    Plasmid
    #53732
    Purpose
    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    The sigma 70 moiety carries the D581G substitution
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pDULE-ABK

    Plasmid
    #49086
    Purpose
    A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments
    Depositor
    Peter Schultz
    Article
    Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.
    Inserts
    tRNA synthetase
    tRNA for a photocrosslinking amino acid
    Use
    Tags
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    GlnRS and lpp
    Available since
    Nov. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    49086-plasmid-map-sequence-id-396431

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  20. pKB238

    Plasmid
    #170033
    Purpose
    Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Neuropeptide Y (NPY Human, Synthetic, Escherichia coli)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170033-plasmid-map-sequence-id-336781

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Showing: 81 - 100 of 194 results