Showing: 81 - 100 of 16418 results
-
Plasmid#45338DepositorTypeEmpty backboneUseC. glabrataTagsExpressionMutationPromoterMET3AvailabilityAcademic Institutions and Nonprofits only
-
pET28-MetN
Plasmid#114458PurposeExpression of hexahistidine-tagged AceS methylase in E. coliDepositorInsertaceS
UseTagsHexahistidineExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
GFP-LiMETER-v1
Plasmid#113933PurposeExpress an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFPDepositorInsertLOV2 and Rit polybasic domain (PB)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
GFP-LiMETER-v2
Plasmid#113934PurposeAlso termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFPDepositorInsertLOV2 and Stim1 polybasic domain (PB)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
WT MetNIQ in pBAD
Plasmid#118253PurposeWild Type Methionine Binding Protein and Methionine ABC Transporter in pBAD Vector for Transport Activity AssayDepositorUseTags10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…ExpressionBacterialMutationChanged Glutamic Acid (Glu) 295 to Alanine (Ala)PromoterNone and araBAD PromoterAvailabilityAcademic Institutions and Nonprofits only -
WT MetNI in pBAD
Plasmid#118254PurposeWild Type Methionine ABC Transporter MetNI in pBAD Vector for Transport Activity AssayDepositorUseTags10x His Tag and Enterokinase Cleavage SiteExpressionBacterialMutationPromoterArabinose Promoter and NoneAvailabilityAcademic Institutions and Nonprofits only -
N229A Lo-MetQ
Plasmid#118268PurposeN229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structureDepositorInsertMetQ (N229A) (metQ E. coli)
UseTags10x His Tag and Enterokinase Cleavage SiteExpressionBacterialMutationChanged Asparagine (Asn) 229 to Alanine (Ala)PromoterT7 PromoterAvailabilityAcademic Institutions and Nonprofits only -
pICE MET17
Plasmid#122880PurposeMET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Met1ILeu ELL2
Plasmid#127258PurposeFor in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met1 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet1 to Ileu by QuikchangePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Met133ILeu ELL2
Plasmid#127259PurposeFor in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met133 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet133 to Ileu QuikchangePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Met138ILeu ELL2
Plasmid#127260PurposeFor in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met138 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet138 to Ileu QuikchangePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Met186ILeu ELL2
Plasmid#127261PurposeFor in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met186 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet186 to Ileu QuikchangePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Met331Leu ELL2
Plasmid#127262PurposeFor in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met331 to Ileu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet331 to ILeu QuikchangePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pMET17p-CRU313
Plasmid#131163PurposeFor ScMET17 promoter regulated expression of Cub-R-URA3 fused bait proteins, centromeric ARS plasmid, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutantDepositorTypeEmpty backboneUseTagsCRUExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
NmMetQ N238A
Plasmid#129648PurposeExpresses N238A mutation in the mature metQ gene from Neisseria meningitides, without the signal sequenceDepositorInsertmetQ Gene
UseTags10x Histidine Tag, Enterokinase, and pelB signal …ExpressionBacterialMutationChanged Asparagine 238 to AlaninePromoterT7 PromoterAvailabilityAcademic Institutions and Nonprofits only -
pENTR-c-Met
Plasmid#31786DepositorInsertMet (MET Human)
UseEntry vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-Mettl4
Plasmid#86665PurposeExpresses murine Mettl4 containing N-terminal 3XFlag tagDepositorInsertMettl4 (Mettl4 Mouse)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
p426MET25_sfpHluorin (MRV55)
Plasmid#115697Purposesuperfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)DepositorInsertsfpHluorin
UseTagsExpressionYeastMutationfor introduced mutations see publicationPromoterMET25AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-metap2
Plasmid#100512PurposeExpresses HA tagged metap2 in mammalian cellsDepositorInsertMetap2 (METAP2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCDH-METTL14-WT
Plasmid#141112PurposeOverexpression of WT METTL14 in mammalian cells.DepositorInsertHMETTL14 (METTL14 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 81 - 100 of 16418 results