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Plasmid#51565PurposeExpression of bacteriophage lambda CI for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertcI
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFS119
Plasmid#12379DepositorInsertade6, thymidine kinase
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMal-MATa1
Plasmid#8436DepositorInsertMATa1(78aa-126aa)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMal-EcPhr
Plasmid#136244PurposeProtein expression of E. coli PhrDepositorInsertEcPhr
UseTagsMBPExpressionBacterialMutationV115E- Please see depositor comments belowPromoterAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFS118
Plasmid#12378DepositorInsertura4, thymidine kinase
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCVD30
Plasmid#63691PurposepCVD25 with the Tcr gene removed, XbaI is intact, 540 bp of CT A is deleted. Cloned from V. cholerae 395 chromosome.DepositorInsertV. cholera chromosomal DNA
UseTagsExpressionBacterialMutation540 bp of CT A is deletedPromoterAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pMBP-MS2
Plasmid#65104Purposecan be used to isolate RNA binding complexesDepositorInsertMS2
UseTagsMBPExpressionBacterialMutationdouble mutation (V75Q and A81G) that prevents oli…PromotertacAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI-J23106-geneIII
Plasmid#80843PurposePhagemid (encodes M13 gene III and lambda cIopt).DepositorInsertsM13 gene III
Lambda cI
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
h3.7SP-Crtta
Plasmid#20180DepositorInserth3.7SP-Crtta (SFTPC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cIopt-J23106-geneIII
Plasmid#80852PurposePhagemid (encodes M13 gene III and lambda cIopt).DepositorInsertsM13 gene III
Lambda cIopt
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
MT2a
Plasmid#105693PurposeBacterial expression plasmids for production of recombinant metallothionein 2aDepositorInsertMetallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMal-E4rGel
Plasmid#51280PurposeExpresses a fibronectin fusion with gelonin targeting EGFR in bacteriaDepositorInsertE4rGel
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pH1v1
Plasmid#60244PurposegRNA expression by the H1 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterH1 promoterAvailable sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5C6A-J23106-geneIII
Plasmid#80905PurposePhagemid (encodes cI5C6A and M13 gene III)DepositorInsertsM13 gene III
cI5C6A
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CRP/CAP
Plasmid#51563PurposeExpression of E. coli CRP/CAP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backboneDepositorInsertCRP/CAP
UseTagsIntein-Chiting Binding DomaninExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5G6G-J23106-geneIII
Plasmid#80906PurposePhagemid (encodes cI5G6G and M13 gene III)DepositorInsertsM13 gene III
cI5G6G
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5G6T-J23106-geneIII
Plasmid#80907PurposePhagemid (encodes cI5G6T and M13 gene III)DepositorInsertsM13 gene III
cI5G6T
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5T6T-J23106-geneIII
Plasmid#80908PurposePhagemid (encodes cI4A5T6T and M13 gene III)DepositorInsertsM13 gene III
cI4A5T6T
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF_dCas9
Plasmid#68416PurposeTransient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.DepositorInsertdCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A/H841APromoterhuman EF1[alpha]Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only