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Showing: 81 - 100 of 44815 results

  1. Flag_MmPERK_S503_N1114_pBabePuro

    Plasmid
    #58423
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant mouse PERK deleted from amino acids M1 to Y502. The remaining amino acids are S503 to N1114.
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    PERK (Eif2ak3 Mouse)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    Deleted amino acids M1 to Y502
    Promoter
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    58423-plasmid-map-sequence-id-123700

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  6. pMSCV_puro_41584

    Plasmid
    #111630
    Purpose
    Complementation of Dicer_KO mESCs with wild type human DICER isoform
    Depositor
    Constance Ciaudo
    Insert
    FLAG-hsDicer (DICER1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    111630-plasmid-map-sequence-id-217596

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  7. pMSCV_puro_41585

    Plasmid
    #111631
    Purpose
    Complementation of Dicer_KO mESCs with mutated human DICER isoform
    Depositor
    Constance Ciaudo
    Insert
    FLAG-hsDicer (D1320A/D1709A) (DICER1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D1320A/D1709A
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111631-plasmid-map-sequence-id-217672

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  13. pMSCV_puro_41589

    Plasmid
    #111632
    Purpose
    Complementation of Dicer_KO mESCs with mutated human DICER isoform
    Depositor
    Constance Ciaudo
    Insert
    FLAG-hsDicer (K70A) (DICER1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K70A
    Promoter
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    111632-plasmid-map-sequence-id-217766

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  14. AAV2_hSyn_Aurora_CA_Citrine

    Plasmid
    #98219
    Purpose
    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Aurora_C128A gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…
    Promoter
    human synapsin
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98219-plasmid-map-sequence-id-256052

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  18. lentiGuide-Puro

    Plasmid
    #52963
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Cloning Grade DNA
    Inserts
    S. pyogenes sgRNA cassette
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-a and hU6
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52963-plasmid-map-sequence-id-331247

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Showing: 81 - 100 of 44815 results