We narrowed to 4,558 results for: Ado;

1. PX458_KMT2B_1

   Plasmid

   #101074

   Purpose

   Encodes gRNA for 3' target of human KMT2B

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against KMT2B (KMT2B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-HyPer-DAAO-NES

   Plasmid

   #119164

   Purpose

   Fusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.

   Depositor

   Thomas Michel

   Article

   Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

   Insert

   HyPer-DAAO-NES
   

   Use

   AAV

   Tags

   NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1a_NRL_P2A_Hygro_Barcode

   Plasmid

   #120469

   Purpose

   Barcoded lentiviral vector to express NRL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   NRL (NRL Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-ihSyn1-DIO-tTA

   Plasmid

   #99121

   Purpose

   An AAV genome with Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed expression of the tTA

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Has Service

   AAV1

   Insert

   DIO-tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   ihSyn1

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDNR-SpCas9-Cdt1

   Plasmid

   #80426

   Purpose

   Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Cdt1
   

   Use

   CRISPR

   Tags

   Cdt1

   Expression

   Mutation

   codon optimized for human

   Promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.4_CEACAM6-His

   Plasmid

   #149337

   Purpose

   for eukaryotic expression and purification of CEACAM6-His6

   Depositor

   James Fleckenstein

   Article

   Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

   Insert

   CEACAM6 (CEACAM6 Human)
   

   Use

   Tags

   IL-2 signal peptide and polyhistidine

   Expression

   Mammalian

   Mutation

   point mutation (GGA to GCA) to replace 'G-g…

   Promoter

   CMV

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-ChRmine-eYFP-WPRE

   Plasmid

   #130988

   Purpose

   AAV vector to drive the expression of ChRmine-eYFP under the control of CamKIIa promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   eYFP

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLPCX2-HA-GATA3 FL

   Plasmid

   #89835

   Purpose

   Express GATA3 in mammalian cells

   Depositor

   Shengyu Yang

   Article

   Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

   Insert

   Gata3 (Gata3 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. EFS-SpdCas9

   Plasmid

   #210703

   Purpose

   This plasmid express EFS promoter driven human codon optimized SpdCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   S. Pyogenes dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a core promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-ADAR2-sesRNA-tTA2-W3SL

    Plasmid

    #192068

    Purpose

    Expression of ADAR2, sesRNA in mammalian cells with hSyn promoter, with tTA2 as efRNA and ADAR2 overexpression.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for mouse Ctip2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MF82_PB-TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

    Plasmid

    #176626

    Purpose

    TF A self-activation cassette in MultiFate-2.2 and MultiFate-3

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5FRT/TO-Strep/HA-UBE2J1

    Plasmid

    #124669

    Purpose

    Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2J1 (UBE2J1 Human)
    

    Use

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTKv10.2

    Plasmid

    #85706

    Purpose

    mammalian expression of Kv10.2

    Depositor

    Luis Pardo

    Article

    Gomez-Varela et al Cancer Res. 2007 Aug 1;67(15):7343-9.

    Insert

    KCNH5 (KCNH5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-ChRmine-eYFP-WPRE

    Plasmid

    #130992

    Purpose

    AAV vector to drive the expression of ChRmine-eYFP under the control of human synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    eYFP

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a_KLF16_P2A_Hygro_Barcode

    Plasmid

    #120502

    Purpose

    Barcoded lentiviral vector to express KLF16 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF16 (KLF16 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a_FOXA3_P2A_Hygro_Barcode

    Plasmid

    #120440

    Purpose

    Barcoded lentiviral vector to express FOXA3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    FOXA3 (FOXA3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pInducer20 TIFA WT

    Plasmid

    #231589

    Purpose

    TIFA WT gene under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    TIFA (TIFA Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    Promoter

    doxycycline

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. AllCB shRNA-1

    Plasmid

    #160065

    Purpose

    Knock Down all Collybistin isoforms

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. canxTM-mEmerald (cytosolic)

    Plasmid

    #186962

    Purpose

    Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CANX (CANX Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Only express calnexin (482-end) and add ER signal…

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRN3P_T3_ABE8e_IVT

    Plasmid

    #201676

    Purpose

    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72

    Insert

    ecTadA(8e)-nSpCas9
    

    Use

    CRISPR; Vector for in-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T3 promoter

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

    Plasmid

    #216763

    Purpose

    PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. px330-CD4

    Plasmid

    #136938

    Purpose

    NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

    Depositor

    Jeremy Stark

    Article

    Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

    Insert

    sgRNA targeting CD4 (CD4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-TRE3G-BE3RA-PGK-Puro

    Plasmid

    #110846

    Purpose

    Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    TRE3G

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV-Ef1a-DIO-ChRmine-eYFP-Kv2.1-WPRE

    Plasmid

    #130997

    Purpose

    Double floxed soma-targeted ChRmine-eYFP under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    EYFP-Kv2.1

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5/FRT/TO-Intron-CterFlag-MePCE

    Plasmid

    #113549

    Purpose

    Mammalian expression plasmid that can be used with the Flp-In T-REx system.

    Depositor

    Blerta Xhemalce

    Article

    Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

    Insert

    MePCE (MEPCE Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Gluc-LHCGR

    Plasmid

    #221781

    Purpose

    Measurement of glycoprotein-glycoprotein receptor binding using the BRET phenomenon and measurement of membrane expression of the Gluc-LHCGR

    Depositor

    Adolfo Rivero-Muller

    Article

    Adamczuk et al Endocrinology. 2024 Apr 29;165(6):bqae05

    Insert

    LHCGR (LHCGR Human)
    

    Use

    Tags

    Gaussia Luciferase and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEE050

    Plasmid

    #176807

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    601
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-hSyn-ClipF-sesRNA-smV5-tTA2-W3SL

    Plasmid

    #192067

    Purpose

    Expression of ClipF, sesRNA in mammalian cells, with smV5 and tTA2 as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for human FXOP2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSUPER-retro-puro-shOrai1

    Plasmid

    #89933

    Purpose

    Knockdown human Orai1 expression

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    Orai1 shRNA (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEE060

    Plasmid

    #176817

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    611
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge