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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,533 results for: Gaa

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Showing: 1471 - 1500 of 4533 results

  3. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide3

    Plasmid
    #118179
    Purpose
    CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118179-plasmid-map-sequence-id-240448

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  4. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide4

    Plasmid
    #118172
    Purpose
    CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118172-plasmid-map-sequence-id-240355

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  5. gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

    Plasmid
    #217342
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Article
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Promoter
    hU6
    Available Since
    April 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    217342-plasmid-map-sequence-id-429351

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  6. gCH198 (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFOLH1-1_crCD151-3_crHBG-3_crKIT-2_crKIT-3_crCD81-1)

    Plasmid
    #217345
    Purpose
    crRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT, CD81
    Depositor
    Luke Gilbert
    Article
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
    crCLTA-4 gRNA: ggctctgcaacaccgcctagacc (CLTA Human)
    crFOLH1-1 gRNA: gctccagacctggggtccagttt (FOLH1 Human)
    crCD151-3 gRNA: cgggaggccgcacccaccgcctg (CD151 Human)
    crHBG-3 gRNA: ttcttcatccctagccagccgcc (HBG1, HBG2 Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Promoter
    hU6
    Available Since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    217345-plasmid-map-sequence-id-426760

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  8. pCGLH-luciferase

    Plasmid
    #194979
    Purpose
    pCGLH control plasmid
    Depositor
    Angelique Bordey
    Insert
    luciferase
    Expression
    Bacterial
    Available Since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    194979-plasmid-map-sequence-id-386135

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  9. pTJK459

    Plasmid
    #138008
    Purpose
    Empty sgRNA expression vector for expression of sgRNA for Sp Cas9
    Depositor
    Tami Kingsbury
    Type
    Empty backbone
    Use
    Lentiviral
    Mutation
    U-A flip and hairpin extension: cgtttAagagctaTGCT…
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138008-plasmid-map-sequence-id-275002

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  10. pSico-shTsg101

    Plasmid
    #194982
    Purpose
    conditional knock down TSG101
    Depositor
    Angelique Bordey
    Insert
    shTsg101
    Expression
    Bacterial
    Available Since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    194982-plasmid-map-sequence-id-386020

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  11. pAAV CAG Barcode3

    Plasmid
    #229064
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    CAG Barcode3
    Use
    AAV
    Available Since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    229064-plasmid-map-sequence-id-455087

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  12. pAAV Syn Barcode15

    Plasmid
    #226189
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode15
    Use
    AAV
    Available Since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    226189-plasmid-map-sequence-id-447880

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  13. pAAV Syn Barcode12

    Plasmid
    #226186
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode12
    Use
    AAV
    Available Since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    226186-plasmid-map-sequence-id-447879

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  14. pAAV Syn Barcode19

    Plasmid
    #226196
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode19
    Use
    AAV
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226196-plasmid-map-sequence-id-447886

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  15. pOpen-EcoRIR

    Plasmid
    #165504
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    EcoRI
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165504-plasmid-map-sequence-id-338511

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  16. mCherry-Ykt6

    Plasmid
    #209888
    Purpose
    Expresses mCherry tagged Ykt6 in mammalian cells
    Depositor
    Geert van den Bogaart
    Insert
    Ykt6
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209888-plasmid-map-sequence-id-413665

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  17. pRNF219.1.0-gDNA

    Plasmid
    #132464
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    RNF219
    Use
    CRISPR
    Available Since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132464-plasmid-map-sequence-id-267084

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  18. lentiCRISPRv2_ZsG gRNA_2

    Plasmid
    #192686
    Purpose
    Lentiviral expression of Cas9 and ZsGreen1 targeting sgRNA
    Depositor
    Jeffrey Miner
    Insert
    Cas9, ZsGreen targeting sgRNA #2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a, U6
    Available Since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    192686-plasmid-map-sequence-id-379590

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  19. FLAG-NM23-H1

    Plasmid
    #25000
    Depositor
    Judy Lieberman
    Insert
    NM23-H1 (NME1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    July 1, 2010
    Availability
    Academic Institutions and Nonprofits only

  20. GOSR1-mCherry

    Plasmid
    #209884
    Purpose
    Expresses human GOSR1 (GS28) fused to mChery in mammalian cells
    Depositor
    Geert van den Bogaart
    Insert
    GOSR1
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209884-plasmid-map-sequence-id-413661

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  22. pOpen-EcoRIM

    Plasmid
    #165515
    Purpose
    Methylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    M.EcoRI
    Use
    Synthetic Biology
    Available Since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165515-plasmid-map-sequence-id-338645

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  23. pTJK644

    Plasmid
    #138012
    Purpose
    SIX1 knockout
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA2 (SIX1 Human)
    Use
    CRISPR and Lentiviral
    Mutation
    None
    Promoter
    U6
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138012-plasmid-map-sequence-id-275045

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  25. pOSR1.1.0-gDNA

    Plasmid
    #176381
    Purpose
    Expresses spCas9 and gRNA sequence targeting OSR1
    Depositor
    Barbara Stranger, Kevin White
    Insert
    OSR1 gRNA
    Use
    CRISPR
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176381-plasmid-map-sequence-id-347393

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  28. pZIC3.1.0-gDNA

    Plasmid
    #176379
    Purpose
    Expresses spCas9 and gRNA sequence targeting ZIC3
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZIC3 gRNA
    Use
    CRISPR
    Available Since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    176379-plasmid-map-sequence-id-347383

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  29. pTJK635

    Plasmid
    #138009
    Purpose
    SIX1 knockout
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA2 (SIX1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    None
    Promoter
    U6
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138009-plasmid-map-sequence-id-275004

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  30. pTJK643

    Plasmid
    #138011
    Purpose
    SIX1 knockout
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA1 (SIX1 Human)
    Use
    CRISPR and Lentiviral
    Mutation
    None
    Promoter
    U6
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138011-plasmid-map-sequence-id-275015

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Showing: 1471 - 1500 of 4533 results