Skip to main content
Addgene
Showing: 981 - 1000 of 19463 results

  6. pCHC023 (∆MBH)

    Plasmid
    #226201
    Purpose
    Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for membrane bound hydrogenase operon
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJ
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226201-plasmid-map-sequence-id-453504

    Enlarge

  8. pCHC027 (∆pemK)

    Plasmid
    #226204
    Purpose
    Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Upstream/downstream homology arms for addiction system toxinPemK.
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226204-plasmid-map-sequence-id-452438

    Enlarge

  12. pCHC025 (∆CBBp)

    Plasmid
    #226203
    Purpose
    Electroporation knockout plasmid (pK18sB) to delete the C. necator megaplasmid copy of the CBB operon (deltaCBBp).
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for megaplasmid copy of the CBB operonthe CBB operon (deltaCBBp).
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ∆cbbR ∆cbbLpSpXpYpEpFpPpTpZpGpKpAp
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226203-plasmid-map-sequence-id-453509

    Enlarge

  13. pCHC036 (∆pHG1)

    Plasmid
    #226206
    Purpose
    Conjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for the pHG1 megaplasmid replication region
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ∆helD ∆repA ∆repB ∆parAB
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226206-plasmid-map-sequence-id-452437

    Enlarge

  14. SGyA

    Plasmid
    #160292
    Purpose
    Drives expression of Cas9 and a TdTomato fluorescent marker
    Depositor
    Omar Akbari
    Article
    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:
    Inserts
    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    Use
    Tags
    T2A GFP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    160292-plasmid-map-sequence-id-316542

    Enlarge

  15. pRS416-Gal4-dCas9-VP64

    Plasmid
    #71128
    Purpose
    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.
    Depositor
    Ronald Davis
    Article
    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.
    Insert
    Gal4-dCas9-VP64
    Use
    Tags
    NLS n-terminal, N terminal Gal4 Activator domain,…
    Expression
    Yeast
    Mutation
    Promoter
    Tef1
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71128-plasmid-map-sequence-id-127063

    Enlarge

  18. pGL3_MSR1_bothneg0topos3pos1

    Plasmid
    #232387
    Purpose
    MSR1 enhancer, left buffering Coordinator converted to sensitizing double Ebox and right buffering Coordinator to sensitizing Coordinator
    Depositor
    Joanna Wysocka
    Insert
    MSR1 (MSR1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    MSR1 enhancer, left buffering Coordinator convert…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232387-plasmid-map-sequence-id-462867

    Enlarge

Showing: 981 - 1000 of 19463 results