We narrowed to 12,666 results for: BASE;

1. pCfB6364

   Plasmid

   #106151

   Purpose

   EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCB-1

   Plasmid

   #210387

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Tags

   Expression

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FRESCA-syntide

   Plasmid

   #132415

   Purpose

   FRET-based sensor for CaMKII activity

   Depositor

   Margaret Stratton

   Article

   Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

   Insert

   FRESCA-syntide
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCB-2

   Plasmid

   #210388

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Tags

   Expression

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBK-VRER-BE3

   Plasmid

   #85173

   Purpose

   Expresses VRER-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   VRER-BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. ND4.2-Left TALE-G1397-N-dead-mCherry

   Plasmid

   #179683

   Purpose

   Expression of base editor in mammalian cells and for cell sorting

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1347A

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ11296: pHR-HSP-hyperdCas12a-miniVPR-EPS-puro

   Plasmid

   #210604

   Purpose

   heat shock promoter - activated hyperdCas12a-based transcriptional activator

   Depositor

   Stanley Qi

   Article

   Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

   Insert

   hyperdCas12a-miniVPR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   truncated hsp70

   Available since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJL-SaKKH-BE3

   Plasmid

   #85170

   Purpose

   Expresses SaKKH-BE3 in mammalian cells [Edit]

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   SaKKH-BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBK-VQR-BE3

   Plasmid

   #85171

   Purpose

   Expresses VQR-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   VQR-BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ND4.2-Left TALE-G1397-N-wildtype

    Plasmid

    #179684

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N_1xUGI_P2A_mCherry_ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ND4.2-Right DdCBE-G1397-C-wildtype

    Plasmid

    #179687

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C )_1xUGI_SOD2 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEN_TmiRc3

    Plasmid

    #25748

    Purpose

    Entry vector for cloning miR30-based shRNA driven by TRE promoter.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-FNLSNG-P2A-Puro

    Plasmid

    #136900

    Purpose

    Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSNG
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

    Promoter

    EF1s

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. negActinin-sstFRET-GR

    Plasmid

    #83417

    Purpose

    FRET-based actinin tension sensor (negative control)

    Depositor

    Tetsuya Kitaguchi

    Article

    Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

    Insert

    negActinin-sstFRET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pH-PABE-7-esgRNA

    Plasmid

    #115620

    Purpose

    Targeted A to G in rice

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-nCas9-3*NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBK-YE1-BE3

    Plasmid

    #85174

    Purpose

    Expresses YE1-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YE1-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-FNLSHiFiNG-P2A-Puro

    Plasmid

    #136903

    Purpose

    Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFiNG
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

    Promoter

    EF1s

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. VinTS-tCRMod-GGSGGS7

    Plasmid

    #111764

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

    Plasmid

    #92121

    Purpose

    CRISPR-Cas9 based efficient genome editing in S. aureus

    Depositor

    Sheng Yang

    Article

    Liu et al Acta Biochim Biophys Sin (Shanghai). 201

    Insert

    Cas9-Pxyltet-sgRNA-pj23119
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ND4.2-Left TALE-G1397-N-DddA6

    Plasmid

    #179680

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (Q1310R S1330I T1380I)_1xUGI_ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q1310R, S1330I, T1380I

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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