We narrowed to 1,889 results for: PTS

1. pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-Resistant

   Plasmid

   #177130

   Purpose

   Generate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Use

   Lentiviral

   Tags

   mClover3

   Expression

   Mutation

   W428A/W377A (m6A binding defects), and siRNA resi…

   Promoter

   Ef1a

   Available since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRSF_his6_sumo_hRNMT_RAM

   Plasmid

   #213528

   Purpose

   Expresses the human RNMT-RAM complex

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Inserts

   human RNMT (RNMT Human)
   RAM
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSF_his6_sumo_SARS_CoV2_NSP14

   Plasmid

   #213518

   Purpose

   Expresses NSP14 of SARS-CoV-2

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   N-terminal His6-sumo

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSF_his6_sumo_OC43_NSP14

   Plasmid

   #213519

   Purpose

   Expresses NSP14 of hCoV OC43

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   hCoV OC43 NSP14 (ORF1ab hCoV OC43)
   

   Use

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRSF_his6_sumo_NL63_NSP14

   Plasmid

   #213520

   Purpose

   Expresses NSP14 of hCoV NL63

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   hCoV NL63 NSP14 (ORF1ab hCoV NL63)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRSF_his6_sumo_229E_NSP14

   Plasmid

   #213521

   Purpose

   Expresses NSP14 of hCoV 229E

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   hCoV 229E NSP14
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTXBI-mClover-YTH domain

   Plasmid

   #177123

   Purpose

   Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTH domain) (YTHDC1 Human)
   

   Use

   Tags

   Mxe GyrA intein-Chitin binding domain for protein…

   Expression

   Bacterial

   Mutation

   YTH domain only (IDR1 and IDR2 deletion)

   Promoter

   T7

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTXBI-mclover-YTHDC1-FL

   Plasmid

   #177121

   Purpose

   Bacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Use

   Tags

   Mxe GyrA intein-Chitin binding domain for protein…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTXBI-mclover-YTH-IDR2

   Plasmid

   #177122

   Purpose

   Bacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTH and IDR2 domains) (YTHDC1 Human)
   

   Use

   Tags

   Mxe GyrA intein-Chitin binding domain for protein…

   Expression

   Bacterial

   Mutation

   YTH and IDR2 domains only (IDR1 deletion)

   Promoter

   T7

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-N1-PHD2

    Plasmid

    #21401

    Depositor

    Eric Metzen

    Article

    Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

    Insert

    EGLN1 (EGLN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pShuttle-FEN1mED

    Plasmid

    #35033

    Depositor

    Sheila Stewart

    Article

    Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

    Insert

    FEN1mWT
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    PT MUTANT E160D

    Promoter

    CMV

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pffDronpa2F-peroxisome

    Plasmid

    #214608

    Purpose

    Expresses reversibly switchable fluorescent protein ffDronpa2F targeted to peroxisome

    Depositor

    Peter Dedecker

    Article

    Valenta et al Talanta. 2024 Mar

    Insert

    ffDronpa2F-peroxisome
    

    Use

    Tags

    peroxisome targeting sequence (PTS)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSKB3.EG10680

    Plasmid

    #11436

    Depositor

    Sung-Hou Kim

    Article

    Shin et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    OsmC (osmC E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A

    Plasmid

    #213527

    Purpose

    Expresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92A

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    NSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    D90A and E92A

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSF_his6_sumo_SARS_CoV2_NSP14_V290A

    Plasmid

    #213522

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation V290A

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed V290 to A

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSF_his6_sumo_SARS_CoV2_NSP14_N306K

    Plasmid

    #213523

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation N306K

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV2-NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed N306 to K

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

    Plasmid

    #213524

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed P335 to S

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

    Plasmid

    #213525

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed P335 to H

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

    Plasmid

    #213526

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed H424 to R

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pM2AH + Slo1

    Plasmid

    #16195

    Depositor

    Larry Salkoff

    Article

    McCobb et al Am J Physiol. 1995 Sep . 269(3 Pt 2):H76

    Insert

    calcium-activated potassium channel (KCNMA1, Kcnma1 Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge