We narrowed to 31,710 results for: Ret;

1. pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Plasmid

   #101201

   Purpose

   Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S701C/S960C
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S701C and S960C

   Promoter

   T7

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Plasmid

   #101200

   Purpose

   Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C and D273C

   Promoter

   T7

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A)

   Plasmid

   #84777

   Purpose

   Second generation lenti expressing Mash1 and Neurogenin for iN transdifferentation to neurons

   Depositor

   Fred Gage

   Article

   Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

   Insert

   Ngn2 and Mash1 with a T2A (NEUROG2, ASCL1 Human)
   

   Use

   Lentiviral

   Promoter

   pTIGHT

   Available Since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Histone H2B targeted Aurora B FRET sensor Kif2 substrate

   Plasmid

   #45216

   Depositor

   Michael Lampson

   Article

   Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

   Insert

   Histone H2B-targeted Aurora B FRET sensor
   

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   May 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

   Plasmid

   #87350

   Purpose

   S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/S355C/C574S/S867C

   Promoter

   T7

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Plasmid

   #101201

   Purpose

   Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S701C/S960C
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S701C and S960C

   Promoter

   T7

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Plasmid

   #101200

   Purpose

   Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C and D273C

   Promoter

   T7

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI

   Plasmid

   #216694

   Purpose

   Synchronize trafficking of GPI-AP from the ER (RUCH system)

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Inserts

   FIREmate-KDEL
   SBP-mApple-FIREtag-GPI
   

   Tags

   FIREtag, IL2 signal sequence, Streptavidin Bindin…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOS012: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

   Plasmid

   #163057

   Purpose

   Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   Grx1-roGFP1-iEER
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

    Plasmid

    #216696

    Purpose

    Synchronize trafficking of TNFalpha from the ER (RUCH system)

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Inserts

    FIREmate-KDEL
    Tumor Necrosis Factor alpha (TNFa)
    

    Tags

    FIREtag, Streptavidin Binding Protein (SBP), and …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

    Plasmid

    #216695

    Purpose

    Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Inserts

    FIREmate-KDEL
    ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
    

    Tags

    FIREtag, Streptavidin Binding Protein (SBP), and …

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Plasmid

    #101211

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Plasmid

    #101204

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRetroX-Tight-Pur-Trim69 Full length, isoform A (codon optimized)

    Plasmid

    #199566

    Purpose

    Stable and dox. inducible expression of Trim69 Full length, isoform A (codon optimized) after retroviral-mediated gene transfer

    Depositor

    Yuxin Song, Andrea Cimarelli

    Article

    Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

    Insert

    Trim69 Full length, isoform A (codon optimized) (TRIM69 Human)
    

    Use

    Retroviral; Allows for puromycin selection

    Tags

    Flag

    Mutation

    codon optimized

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Plasmid

    #101210

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, N497A, R661A, Q695A an…

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

    Plasmid

    #101203

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D13…

    Promoter

    T7

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

    Plasmid

    #101214

    Purpose

    Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/K855A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C and K855A

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Plasmid

    #101212

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S701C, S960C, N497A, R661A, Q695A an…

    Promoter

    T7

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMOS012: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

    Plasmid

    #163057

    Purpose

    Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Grx1-roGFP1-iEER
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Plasmid

    #101211

    Purpose

    Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge