We narrowed to 7,670 results for: URE

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pET-32a(+)-TGFB1

Plasmid

#120361

Purpose

Produce human recombinant TGFB1 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Transforming Growth Factor-β1 (TGFB1 Synthetic, Human)

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 Promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Endosomes-14

Plasmid

#58014

Purpose

Localization: Endosomes, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Endosomes (RHOB Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
3xmNeonGreen_UtrCH

Plasmid

#129607

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Utrophin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-oScarlet (AAV8)

Viral Prep

#137136-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-oScarlet (#137136). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-oScarlet plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of oScarlet. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Promoter

EF1a

Tags

oScarlet (Cre and Flp-dependent)

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only
pDL445

Plasmid

#29338

Depositor

John Taylor

Article

Lazinski et al J Virol. 1993 May . 67(5):2672-80.

Insert

Large form of HDV Delta Antigen

Expression

Mammalian

Available Since

July 27, 2011

Availability

Academic Institutions and Nonprofits only

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Epac-S-H188

Plasmid

#170349

Purpose

mT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Article

Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

Insert

mT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)

Expression

Mammalian

Mutation

Deletion of DEP domain, Catalytically dead T781A …

Promoter

CMV

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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RTA

Plasmid

#64029

Purpose

Expression of Ricin A Chain (RTA) in E. coli

Depositor

Vern Schramm

Article

Ho et al Proc Natl Acad Sci U S A. 2009 Dec 1;106

Insert

Ricin A Chain

Tags

none

Expression

Bacterial

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
FCK-QuasAr2-Citrine

Plasmid

#59172

Purpose

Fluorescent membrane voltage sensor

Depositor

Adam Cohen

Article

Zou et al Nat Commun. 2014 Aug 13;5:4625. doi: 10.

Insert

QuasAr2-Citrine eFRET

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2

Plasmid

#112004

Purpose

For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV9

Insert

axon-GCaMP6s-P2A-mKate2

Use

AAV

Tags

GAP43 palmitoylation domain

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA_mTurquoise2

Plasmid

#108237

Purpose

Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

PsbA promoter - mTurquoise2

Expression

Bacterial

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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Galphai2-mTurquoise2

Plasmid

#69621

Purpose

The heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

Galphai2-mTurquoise2

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG/GFP

Plasmid

#139980

Purpose

expresses GFP under the promoter of CAG. Transgene flanked by AAV2 ITR

Depositor

William Pu

Article

Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

Insert

Green fluorescent protein

Use

AAV

Promoter

CAG

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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FCK-QuasAr2-mOrange2 eFRET

Plasmid

#59173

Purpose

Fluorescent membrane voltage sensor

Depositor

Adam Cohen

Article

Zou et al Nat Commun. 2014 Aug 13;5:4625. doi: 10.

Insert

QuasAr2-mOrange2 eFRET

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO-EGFP-G3BP1-WT

Plasmid

#136003

Purpose

DOX-inducible WT G3BP1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-mTurquoise-MLC-IRES-neo

Plasmid

#85145

Purpose

lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

mTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)

Use

Lentiviral

Tags

mTurquoise

Promoter

EF1a

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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nAChR beta2 YFP

Plasmid

#15107

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

beta2-YFP-M (Chrnb2 Mouse)

Tags

YFP

Expression

Mammalian

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pME-Gal80ts

Plasmid

#51619

Purpose

vertebrate expression of full-length temperature-sensitive Gal80 with stop

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80ts (GAL80 Budding Yeast)

Use

Vertebrate expression

Mutation

temperature sensitive (G203V, G323R)*

Available Since

March 27, 2014

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-TfR

Plasmid

#12130

Depositor

Pamela Bjorkman

Article

Lebron et al Cell. 1998 Apr 3. 93(1):111-23.

Insert

transferrin receptor (TFRC Human)

Tags

6X-His tag, Factor Xa cleavage site, and leader p…

Expression

Insect

Mutation

The portion of the human TfR gene encoding residu…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
Human b2 nicotinic subunit crystallization construct

Plasmid

#85842

Purpose

pEZT-BM vector with beta2 gene after CMV promoter, published crystallization construct

Depositor

Ryan Hibbs

Article

Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do

Insert

Human b2 nAChR subunit crystallization construct (CHRNB2 Human)

Use

Baculovirus (bacmam) production and mammalian exp…

Tags

C-terminal StrepII tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pFLAG-4EHP

Plasmid

#17342

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

4EHP (EIF4E2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only