We narrowed to 17,708 results for: URE

1. UPB-lung (UPB2)

   Plasmid

   #180788

   Purpose

   Unique Projection Barcode (UPB2)-Lung for Projection-seq

   Depositor

   Rui Chang

   Article

   Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

   Insert

   humanized ChR2-H134R (partial)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. UPB-colon (UPB4)

   Plasmid

   #180786

   Purpose

   Unique Projection Barcode (UPB4)-Colon for Projection-seq

   Depositor

   Rui Chang

   Article

   Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

   Insert

   hM3Dq ( partial )
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETSUMO2_Bacteroidetes-bGSDM-TEV

   Plasmid

   #222101

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Bacteroidetes-bGSDM-TEV ORF
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   TEV protease site replaces residues E242–G248

   Promoter

   T7

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSNRW-Z5-Nluc

   Plasmid

   #174487

   Purpose

   Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

   Depositor

   Richard C. Willson

   Article

   Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

   Insert

   Z5-Nluc
   

   Use

   Tags

   6x Histidine tag (N terminal on insert)

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

   Plasmid

   #83485

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R472A_H477A_R1048A_H1053A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R472A, H477A, R1048A, H1053A

   Promoter

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSUPER-retro-puro-tetO-CTR1-shRNA

   Plasmid

   #53180

   Purpose

   Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   CTR1 (SLC31A1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

7. 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

   Plasmid

   #60815

   Purpose

   dCas9 with single cysteine residue (M1C) for site-specific labelling

   Depositor

   Jennifer Doudna

   Article

   O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

   Insert

   dCas9 M1C D10A C80S H840A C574S
   

   Use

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   M1C D10A C80S H840A C574S

   Promoter

   Available Since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635

   Plasmid

   #91918

   Purpose

   Expresses LbfCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LbfCas13a and guide expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTight-9-124

   Plasmid

   #31874

   Depositor

   Jerry Crabtree

   Article

   Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

   Insert

   miR-9/9* and miR-124 (Mir124a-2, Mir9-3 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 3×Cherry Dam AID Smash

    Plasmid

    #227374

    Purpose

    For inducible expression and cytosolic localization of Dam

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    Dam DNA-adenine methyltransferase
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-Flag-Kdm6b(1025-End)-pA

    Plasmid

    #100278

    Purpose

    In vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

    Insert

    Kdm6b (Kdm6b Mouse)
    

    Use

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    catalytic domain (1025aa-End)

    Promoter

    T7

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1 Utr230-EGFP

    Plasmid

    #58472

    Purpose

    Expresses a cytoplasmic actin filament reporter, a truncation of the tandem CH domains (a.a. 1-230) from human utrophin, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Bioarchitecture. 2015 Aug 28:1-14.

    Insert

    Utr230 (UTRN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1 Utr261-EGFP

    Plasmid

    #58471

    Purpose

    Expresses a cytoplasmic actin filament reporter, the tandem CH domains (a.a. 1-261) from human utrophin, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Bioarchitecture. 2015 Aug 28:1-14.

    Insert

    Utr261 (UTRN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSV-CaMKII

    Plasmid

    #45064

    Purpose

    Expression of CaMKII

    Depositor

    Richard Maurer

    Article

    Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

    Insert

    CaM Kinase II (Camk2a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPK2301

    Plasmid

    #65778

    Purpose

    Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    St1Cas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBB75-ncRNA

    Plasmid

    #194087

    Purpose

    Expresses Ec86 ncRNA in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 ncRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSP977

    Plasmid

    #65774

    Purpose

    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E mutation in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC029 - Lw2Cas13a from Leptotrichia wadei F0279

    Plasmid

    #91919

    Purpose

    Expresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    Lw2Cas13a and guide expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21b-rrt-His8

    Plasmid

    #194088

    Purpose

    Expresses Ec86 reverse transcriptase in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 rrt
    

    Use

    Tags

    His8

    Expression

    Mutation

    Promoter

    T7

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXspuro-GFP-NBR1-dUBA

    Plasmid

    #74205

    Purpose

    Retroviral expression of GFP-NBR1 with aa 877 mutation to generate a premature stop codon

    Depositor

    Jayanta Debnath

    Article

    Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

    Insert

    neighbor of BRCA1 gene 1 (NBR1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed aa 877 to encode for premature stop codon

    Promoter

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only