We narrowed to 1,580 results for: cas12a

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pMaCTag-P32

Plasmid

#120043

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-Tag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

S-Tag

Use

Pcr template

Tags

S-Tag

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-29

Plasmid

#120008

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with Strep-II

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Strep-II

Use

Pcr template

Tags

Strep-II

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-crRNA4n96

Plasmid

#123640

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

crRNA scaffold 4n96

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ11444

Plasmid

#183628

Purpose

pHR-R(AP1-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

Cas12 Guide (crGFP*)

Use

CRISPR and Lentiviral

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMaCTag-11

Plasmid

#119990

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet-i

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-24

Plasmid

#120003

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with TAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TAP

Use

Pcr template

Tags

TAP

Promoter

None

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z18

Plasmid

#120061

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKeima8.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKeima8.5

Use

Pcr template

Tags

mKeima8.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-Z16

Plasmid

#120059

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Dronpa3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Dronpa3

Use

Pcr template

Tags

Dronpa3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z04

Plasmid

#120047

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mTFP1

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mTFP1

Use

Pcr template

Tags

mTFP1

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P20

Plasmid

#120031

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 10xSuntag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

10xSuntag

Use

Pcr template

Tags

10xSuntag

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P03

Plasmid

#120014

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxCerulean3

Use

Pcr template

Tags

moxCerulean3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-27

Plasmid

#120006

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 1xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

1xHA

Use

Pcr template

Tags

1xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-14

Plasmid

#119993

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeptune2.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeptune2.5

Use

Pcr template

Tags

mNeptune2.5

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-10

Plasmid

#119989

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mKOk

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKOk

Use

Pcr template

Tags

mKOk

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-08

Plasmid

#119987

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mClover3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mClover3

Use

Pcr template

Tags

mClover3

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-33

Plasmid

#119986

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with FLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

FLAG

Use

Pcr template

Tags

FLAG

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-01

Plasmid

#119980

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with moxBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxBFP

Use

Pcr template

Tags

moxBFP

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ8637 PB-PGK-C intein-2XNLS-miniVPR-mCherry-pA

Plasmid

#140224

Purpose

Constitutive PGK driven expression of intein with miniVPR for 3-way split activator

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

C-intein with miniVPR

Tags

mCherry

Expression

Mammalian

Promoter

PGK

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ131C-RZ-Lb

Plasmid

#134347

Purpose

Golden Gate entry vector; empty vector to clone the 1st LbCas12a crRNA under OsU6 promoter

Depositor

Yiping Qi

Article

Li et al Plant Biotechnol J. 2019 Nov 15. doi: 10

Type

Empty backbone

Use

CRISPR

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pRDA_763

Plasmid

#201156

Purpose

EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' NLS for CRISPRa

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

5x-ALFA tag-dEnAsCas12a

Use

CRISPR and Lentiviral

Mutation

D908A

Promoter

EFS

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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