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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,112 results for: cas12a

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Showing: 981 - 1000 of 1112 results

  1. pY111 (pcDNA3.1-huTsCpf1)

    Plasmid
    #92267
    Purpose
    Expression of humanized TsCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huTsCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    July 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92267-plasmid-map-sequence-id-183046

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  4. pCSN069

    Plasmid
    #166728
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Publication
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-Mxi1
    Use
    CRISPR
    Tags
    Mxi1
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166728-plasmid-map-sequence-id-332209

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  5. pJK526

    Plasmid
    #155387
    Purpose
    PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA4 in dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    155387-plasmid-map-sequence-id-309008

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  7. pY126 (pcDNA3.1-huBoCpf1)

    Plasmid
    #92302
    Purpose
    Expression of humanized BoCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huBoCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92302-plasmid-map-sequence-id-181630

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  8. pY120 (pcDNA3.1-huFbCpf1)

    Plasmid
    #92296
    Purpose
    Expression of humanized FbCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huFbCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92296-plasmid-map-sequence-id-181502

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  9. pY113 (pcDNA3.1-huPb2Cpf1)

    Plasmid
    #92272
    Purpose
    Expression of humanized Pb2Cpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huPb2Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92272-plasmid-map-sequence-id-181492

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  12. pAsCpf1GG

    Plasmid
    #131011
    Purpose
    Backbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.
    Depositor
    Chase Beisel
    Publication
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of AsCas12a
    GFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131011-plasmid-map-sequence-id-258106

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  13. pEPOR1CB0013

    Plasmid
    #117549
    Purpose
    Level 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Publication
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
    Expression
    Plant
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117549-plasmid-map-sequence-id-228087

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  15. pY123 (pcDNA3.1-huCMaCpf1)

    Plasmid
    #92299
    Purpose
    Expression of humanized CMaCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huCMaCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92299-plasmid-map-sequence-id-181633

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  16. pY125 (pcDNA3.1-huBfCpf1)

    Plasmid
    #92301
    Purpose
    Expression of humanized BfCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huBfCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92301-plasmid-map-sequence-id-181503

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  17. pY119 (pcDNA3.1-huLb5Cpf1)

    Plasmid
    #92295
    Purpose
    Expression of humanized Lb5Cpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huLb5Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92295-plasmid-map-sequence-id-181498

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  18. pY115 (pcDNA3.1-huMlCpf1)

    Plasmid
    #92275
    Purpose
    Expression of humanized MlCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huMlCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92275-plasmid-map-sequence-id-181494

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  19. pKS7107

    Plasmid
    #89051
    Purpose
    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9
    Depositor
    Ervin Welker
    Publication
    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Nm crRNA
    Nm tracrRNA
    hNmCas9
    Use
    CRISPR
    Tags
    HA tag, NLS, and SV40 NLS
    Promoter
    EF1a and human U6
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    89051-plasmid-map-sequence-id-176334

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  20. pYPQ230-D156R

    Plasmid
    #188542
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editing
    Depositor
    Yiping Qi
    Publication
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-D156R
    Use
    CRISPR; Gateway compatible lbcas12a-d156r entry c…
    Tags
    NLS
    Expression
    Plant
    Mutation
    D156R
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    188542-plasmid-map-sequence-id-369465

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Showing: 981 - 1000 of 1112 results