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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,063 results for: golden gate assembly

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Showing: 981 - 1000 of 1063 results

  1. pYSD894

    Plasmid
    #253020
    Purpose
    A MoClo YTK Type 3 part GFP dropout vector to assemble a signal peptide and protein of interest as N-terminal fusion with 2xFLAG-Aga2 display anchor in a Met15, CEN6/ARS4 yeast expression plasmid
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    GFP
    Expression
    Yeast
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253020-plasmid-map-sequence-id-508728

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  2. pYSD892

    Plasmid
    #253018
    Purpose
    A MoClo YTK Type 3 part GFP dropout vector to assemble a signal peptide and protein of interest as N-terminal fusion with 2xFLAG-Aga1 del N181aa anchor in a HIS3, CEN6/ARS4 yeast expression plasmid
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    GFP
    Expression
    Yeast
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253018-plasmid-map-sequence-id-508727

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  3. pYSD891

    Plasmid
    #253017
    Purpose
    A MoClo YTK Type 3 part GFP dropout vector to assemble a signal peptide and protein of interest as N-terminal fusion with 2xFLAG-649 stalk display anchor in a LEU2, CEN6/ARS4 yeast expression plasmid
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    GFP
    Expression
    Yeast
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253017-plasmid-map-sequence-id-508726

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  4. pYSD893

    Plasmid
    #253019
    Purpose
    A MoClo YTK Type 3 part GFP dropout vector to assemble a signal peptide and protein of interest as N-terminal fusion with 2xFLAG-SED1 display anchor in a URA3, CEN6/ARS4 yeast expression plasmid
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    GFP
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253019-plasmid-map-sequence-id-507368

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  7. pPD1178-LPDV promoterless PuroR-P2A-miRFP720

    Plasmid
    #201537
    Purpose
    Landing pad destination vector for mMoClo golden gate-based assembly of landing pad integration vectors; contains BxB1 attB site, promoterless puromycin resistance gene and miRFP720 gene
    Depositor
    Joshua Leonard
    Article
    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:
    Insert
    Promoterless PuroR-P2A-miRFP720
    Expression
    Mammalian
    Promoter
    None
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    201537-plasmid-map-sequence-id-401330

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  19. pNHT7

    Plasmid
    #186753
    Purpose
    A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.
    Depositor
    Bo Wang
    Article
    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002
    Type
    Empty backbone
    Use
    Synthetic Biology; Cloning backbone
    Promoter
    T7
    Available Since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186753-plasmid-map-sequence-id-375095

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Showing: 981 - 1000 of 1063 results