We narrowed to 1,255 results for: post

1. pmirGlo-CALB2-3'UTR mir-30 mut1

   Plasmid

   #74428

   Purpose

   Calb2 UTR with mutated mir-30 site 1

   Depositor

   Rolf Stahel, Emanuela Felley-Bosco

   Article

   Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

   Insert

   CALB2 3'UTR (CALB2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmirGlo-CALB2-3'UTR delta ARE

   Plasmid

   #74425

   Purpose

   Calb2 UTR with mutated AU binding sequence

   Depositor

   Rolf Stahel, Emanuela Felley-Bosco

   Article

   Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

   Insert

   CALB2 3'UTR (CALB2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiGuide Blast

   Plasmid

   #199622

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

   Depositor

   Danwei Huangfu

   Article

   Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-tetO-NGN2

   Plasmid

   #197090

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTriEx-RhoA-wt_mScarlet-i_SGFP2

   Plasmid

   #85071

   Purpose

   Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
   

   Use

   Tags

   6xHis and mScarlet-i and SGFP2

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. hETFDH CRISPR_1

   Plasmid

   #232309

   Purpose

   Human ETFDH Gene Knockout (gRNA 1)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   ETFDH-targeting gRNA (ETFDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEtfdh CRISPR_2

   Plasmid

   #232313

   Purpose

   Mouse Etfdh Gene Knockout (gRNA 2)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   Etfdh-targeting gRNA (Etfdh Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEtfdh CRISPR_1

   Plasmid

   #232312

   Purpose

   Mouse Etfdh Gene Knockout (gRNA 1)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   Etfdh-targeting gRNA (Etfdh Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. hETFDH CRISPR_2

   Plasmid

   #232310

   Purpose

   Human ETFDH Gene Knockout (gRNA 2)

   Depositor

   Ivan Topisirovic

   Article

   Papadopoli et al bioRxiv 2024.10.25.620155

   Insert

   ETFDH-targeting gRNA (ETFDH Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHDR_sox2HA_P2A-GFP-pA_HA_G2913A

    Plasmid

    #196191

    Purpose

    Tagging human SOX2 gene with P2A-EGFP at the C-terminus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    SOX2-HAL-P2A-EGFP-SOX2-HAR (SOX2 Human)
    

    Use

    Homology repair donor

    Tags

    Expression

    Mutation

    G2913A mutation to replace the guide cutting site…

    Promoter

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFPC2-Gephyrin P1

    Plasmid

    #68815

    Purpose

    expression of fluorescent tagged Geph in mammalian cells

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silent mutations on internal EcoRI site. Gephyri…

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS

    Plasmid

    #145027

    Purpose

    Lentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear marker

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    3xnls-Tq-Ca-FLITS
    

    Use

    Lentiviral

    Tags

    3xnls-Tq-Ca-FLITS and H2B-Maroon

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR3-FLAG-Gephyrin P1

    Plasmid

    #68816

    Purpose

    Flag tagged Geph mammalian expression

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    silent mutations on internal EcoRI site. Gephyrin…

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pIRESh-GFPII-SM-Dlk1

    Plasmid

    #108932

    Purpose

    GFP-tagged vector expressing full length isoform of Dlk1

    Depositor

    Anne Ferguson-Smith

    Article

    Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

    Insert

    Dlk1 (Dlk1 Mouse)
    

    Use

    Tags

    GFPII

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

    Plasmid

    #85072

    Purpose

    Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    RhoA Biosensor constitutively active (Q63L) (RHOA Human)
    

    Use

    Tags

    6xHis and mScarlet-i and SGFP2

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q63L

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Mouse Alpha2 delta-3 pMT2

    Plasmid

    #58727

    Purpose

    expression of mouse Alpha2 delta-3 in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

    Insert

    alpha2 delta-3 (Cacna2d3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MBP-rPKM2 WT

    Plasmid

    #107158

    Purpose

    MBP-rPKM2 WT for production and purification of rat PKM2 WT

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Mouse Alpha2 delta-3 HA pMT2

    Plasmid

    #58728

    Purpose

    expression of HA tagged mouse Alpha2 delta-3 in mamalian cells

    Depositor

    Annette Dolphin

    Article

    Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

    Insert

    alpha2 delta-3 (Cacna2d3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZE21/UBP1/ClpS

    Plasmid

    #98566

    Purpose

    Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon

    Depositor

    George Church

    Article

    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

    Inserts

    Truncated ubiquitin cleavase, codon optimized
    ATP-dependent Clp protease adapter protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    In a synthetic operon downstream of UBP1 and Remo…

    Promoter

    Same as UBP1 (operon) and Tet promoter (aTc induc…

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFPC2-gephyrin S268A/270A

    Plasmid

    #199608

    Purpose

    Encodes N-terminal eGFP-tagged P1-gephyrin S268/270A for expression in mammalian cells.

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S268A/S270A

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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