We narrowed to 28,641 results for: Tat

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MBP-hnRNPA2_FL_WT

Plasmid

#98662

Purpose

Expresses MBP-tagged full length hnRNPA2

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis-MBP

Expression

Bacterial

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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GFP KNL1 wt hardened and RVSF->AAAA

Plasmid

#45225

Depositor

Iain Cheeseman, Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

GFP KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA (KNL1 Human)

Mutation

RVSF mutated to AAAA

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only
tetO-FOSL1

Plasmid

#170687

Purpose

doxycycline-inducible overexpression of FOSL1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

FOSL1 (FOSL1 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pegRNA-HEK3_del1-5-NGG

Plasmid

#185478

Purpose

pegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.

Depositor

Sabine Fuchs

Article

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

HEK3_del1-5-NGG pegRNA

Expression

Mammalian

Promoter

U6

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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p2K7-bsd-UBI-tagRFP-KDEL

Plasmid

#114179

Purpose

Lentiviral vector for expression of tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

tagRFP-KDEL

Use

Lentiviral

Tags

KDEL retention signal and signal peptide

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Ufd1-Strep-HA

Plasmid

#113474

Purpose

Expression of human Ufd1 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

Ufd1 (UFD1 Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES proHB-EGF NC

Plasmid

#11604

Depositor

Rosalyn Adam

Article

Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

Insert

HB-EGF (HBEGF Human)

Expression

Mammalian

Mutation

Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

Plasmid

#133794

Purpose

U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

NmeCas9 and Nme2Cas9 sgRNA entry vector

Expression

Mammalian

Mutation

sgRNA architecture from Amrani et al. Genome Biol…

Promoter

U6

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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2xMARS

Plasmid

#205233

Purpose

Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cells

Depositor

Jeremy Baskin

Article

Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816

Insert

two repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)

Tags

Nuclear Export Sequence and mScarlet-i

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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G-GECO1.2-Orai1

Plasmid

#73562

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

G-GECO1.2-Orai1 (ORAI1 Human, Synthetic)

Tags

G-GECO1.2

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2A-Qa1 SCT-F2A-neo-AAVS1

Plasmid

#205445

Purpose

human AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse CD59, and Qa1 SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

iCasp9-Crry-mCD59-Qa1 SCT

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[1,2]-C (TRP1)

Plasmid

#177795

Purpose

Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[1,2]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-hDDB2-WT-V5

Plasmid

#167468

Purpose

ectopic expression of hDDB2 in mammalian cells

Depositor

Steven Artandi

Article

Chen et al DNA Repair (Amst). 2021 Jan;97:103022. d

Insert

DDB2 (DDB2 Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES proHB-EGF CS

Plasmid

#11603

Depositor

Rosalyn Adam

Article

Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

Insert

HB-EGF (HBEGF Human)

Expression

Mammalian

Mutation

Constitutively secreted HB-EGF: C-terminal amino …

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pOTTC385 - pAAV CMV-IE IRES EGFP

Plasmid

#102936

Purpose

An AAV vector that expresses IRES EGFP under the CMV-IE promoter

Depositor

Brandon Harvey

Article

Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

CMV-IE

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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HDM_Spikedelta21_sinobiological_B.1.617.2

Plasmid

#204149

Purpose

Expression plasmid for SARS-CoV-2 Delta variant spike

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

SARS-CoV-2 Delta variant spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

21 amino acid cytoplasmic tail deletion

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC946 - pAAV CMV-IE NK1R-IRES-EGFP

Plasmid

#102935

Purpose

An AAV vector that expresses rat NK1R-IRES-EGFP under the CMV-IE promoter

Depositor

Brandon Harvey

Article

Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

Insert

NK1R (Tacr1 Rat)

Use

AAV

Expression

Mammalian

Promoter

CMV-IE

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLX302_HOXB13

Plasmid

#70089

Purpose

Expression of human HOXB13 from CMV promoter, puromycin selection marker

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS-hPhyBY276H-mCherry-HRasCT

Plasmid

#100540

Purpose

Expresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

Depositor

Kazuhiro Aoki

Article

Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

Insert

Phytochrome B (1-908 aa, Y276H mutation) (PHYB Mustard Weed)

Tags

mCherry fused with HRas C-terminus for plasma mem…

Expression

Mammalian

Mutation

Human codon optimized PhyB (1-908 aa), Y276H muta…

Promoter

CAG promoter

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pWAP-HGF

Plasmid

#83503

Purpose

Generation of transgenic mice overexpressing the HGF under the control of the WAP gene promoter

Depositor

Marta Gallego, Lothar Hennighausen

Article

Gallego et al Oncogene. 2003 Nov 20;22(52):8498-508.

Insert

HGF (Hgf Mouse)

Use

Mouse Targeting

Mutation

also contains beta-globin sequences from pUC198

Promoter

mouse WAP (whey acidic protein)

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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