Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 42,501 results for: Tat

Clear keywords
Showing: 9981 - 10000 of 42501 results

  1. pLKO-puro-hPPM1A

    Plasmid
    #185382
    Purpose
    For mammalian expression of shRNA: AGGGTAATGGGTTGCGATATG that targets human PPM1A
    Depositor
    Ayaz Najafov
    Insert
    PPM1A (PPM1A Human)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185382-plasmid-map-sequence-id-363721

    Enlarge

  2. mCherry-Paxillin-KM-N-18

    Plasmid
    #55115
    Purpose
    Localization: Focal Adhesions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Paxillin KM
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Mutation to remove Kozak sequence
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. mApple-Paxillin-KM-N-18

    Plasmid
    #54936
    Purpose
    Localization: Focal Adhesions, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Paxillin-KM
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Mutation to remove Kozak sequence
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pICSL30068

    Plasmid
    #245570
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Glutathione S-transferases (GST) + C3
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245570-plasmid-map-sequence-id-495494

    Enlarge

  5. pAc5.1B-EGFP-DmAIR1_AA

    Plasmid
    #148199
    Purpose
    Insect Expression of DmAIR1
    Depositor
    Elisa Izaurralde
    Insert
    DmAIR1 (l(1)air1 Fly)
    Expression
    Insect
    Mutation
    9 non-silent mutations and 5 silent mutations com…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148199-plasmid-map-sequence-id-290647

    Enlarge

  6. pAc5.1B-lambdaN-HA-DmAIR1_AA

    Plasmid
    #148206
    Purpose
    Insect Expression of DmAIR1
    Depositor
    Elisa Izaurralde
    Insert
    DmAIR1 (l(1)air1 Fly)
    Expression
    Insect
    Mutation
    9 non-silent mutations and 5 silent mutations com…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148206-plasmid-map-sequence-id-290654

    Enlarge

  7. mVenus-Cx26-M-7

    Plasmid
    #56608
    Purpose
    Localization: Gap Junctions, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    Cx26
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    silent mutation in the untranslated sequence; GCT
    Promoter
    CMV
    Available Since
    Dec. 19, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. mVenus-Paxillin-KM-N-18

    Plasmid
    #56334
    Purpose
    Localization: Focal Adhesions, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    Paxillin KM
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Mutation to remove Kozak sequence
    Promoter
    CMV
    Available Since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

    Enlarge

  15. YFP(1-158)-beta-1 in pcDNAI/Amp

    Plasmid
    #54468
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Insert
    YFP (1-158)/beta-1 (GNB1 Human, Aequorea victoria)
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-1 was amplified via PCR, which removed an i…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54468-plasmid-map-sequence-id-112586

    Enlarge

  16. YFP(1-158)-beta-2 in pcDNAI/Amp

    Plasmid
    #54469
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-2. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Insert
    YFP(1-158)/beta-2 (GNB2 Human, Aequorea victoria)
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54469-plasmid-map-sequence-id-114883

    Enlarge

  17. YFP(1-158)-beta-5 in pcDNAI/Amp

    Plasmid
    #54470
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Insert
    YFP(1-158)/beta-5 (GNB5 Human, Aequorea victoria)
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54470-plasmid-map-sequence-id-114865

    Enlarge

  18. pcDNA3.1(+) Laccase2 MCS-mFndc3b Mutant miRNAs

    Plasmid
    #126444
    Purpose
    Expresses a mutated version of mouse Fndc3b circular RNA with the miR-93-3p, miR-258-5p, and miR-412-3p target sites all mutated
    Depositor
    Jeremy Wilusz
    Article
    Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:
    Insert
    Fndc3b (Fndc3b Mouse)
    Expression
    Mammalian
    Mutation
    circFndc3b exons are separated by a miniature int…
    Promoter
    CMV
    Available Since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126444-plasmid-map-sequence-id-245088

    Enlarge

  19. CFP(159-238)-beta-1 in pcDNAI/Amp

    Plasmid
    #55592
    Purpose
    A carboxyl-terminal CFP fragment was fused to Gbeta-1. When co-expressed with an amino-trerminal CFP or YFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    CFP (159-238)-Beta 1 (GNB1 Human, Aequorea victoria)
    Tags
    CFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    CFP(159-238) includes a substitution of His for A…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55592-plasmid-map-sequence-id-114863

    Enlarge

  20. pT7CFE1-Nhis-GST-CHA-YTH domain (YTHDC1, 317-532)

    Plasmid
    #102277
    Purpose
    For expression of His-GST-tagged human YTHDC1 YTH domain using mammalian cell-free protein expression system (1-step human high-yield mini IVT kit, cat. #88890, Thermo Fisher Scientific)
    Depositor
    Samie Jaffrey
    Article
    Kan et al Nat Commun. 2017 Jul 4;8:15737. doi: 10.
    Insert
    YTH domain of human YTHDC1 (317-532) (YTHDC1 Human)
    Use
    For mammalian cell-free protein expression system…
    Tags
    GST, HA, and HIS
    Promoter
    T7
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    102277-plasmid-map-sequence-id-197159

    Enlarge

Showing: 9981 - 10000 of 42501 results