165,839 results

1. pMSCV-IRES-mCherry FP

   Plasmid

   #52114

   Purpose

   a bicistronic IRES-mCherry containing retroviral vector

   Depositor

   Dario Vignali

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-sgRNA(MS2)

   Plasmid

   #102560

   Purpose

   Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_336

   Plasmid

   #179095

   Purpose

   BE Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   BE3.9 (Cas9-NG)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-Ef1a-CreN-InteinN

   Plasmid

   #187614

   Purpose

   AAV vector that encodes N-terminal portion of split-Intein-mediated split-Cre

   Depositor

   Il Hwan Kim

   Article

   Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

   Insert

   CreN-InteinN
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSIQ

   Plasmid

   #216238

   Purpose

   All-in One IQ transgene switch, empty vector

   Depositor

   Hyunju Ro

   Article

   Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDF-5xT7-TtCsm

   Plasmid

   #128572

   Purpose

   For expression of T. thermophilus Csm in E. coli (Csm1-5)

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

   Inserts

   Csm1
   Csm2, Csm3, Csm4, Csm5
   

   Use

   Tags

   HRV 3C protease cleavage site and 10xHis tag on C…

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli and Cod…

   Promoter

   T7 promoter, lac operator

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TRE-EGFP-EF1a-rtTA

   Plasmid

   #104454

   Purpose

   PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

   Depositor

   Volker Busskamp

   Article

   Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

   Inserts

   enhanced green fluorescent protein
   reverse tetracycline-controlled transactivator 3
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. ABE7.10-F148A

   Plasmid

   #132946

   Purpose

   Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

   Depositor

   Hui Yang

   Article

   Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

   Insert

   ABE7.10(F148A)
   

   Use

   CRISPR; Base editor

   Tags

   Expression

   Mammalian

   Mutation

   ABE7.10(F148A)

   Promoter

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas-CmR(+)

   Plasmid

   #113633

   Purpose

   Chloramphenicol resistant derivative of pCas plasmid

   Depositor

   Olivier Lichtarge

   Article

   Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

   Insert

   Chloramphenicol resistance gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Pcat

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. IFNAR1_pCSdest

    Plasmid

    #53881

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    IFNAR1 (IFNAR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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