We narrowed to 19,405 results for: Tat

Showing: 10081 - 10100 of 19405 results

Results per page:

Sort results by:

pcDNA6-C-Flag-GK5_v2

Plasmid

#134282

Purpose

Expresses Flag-tagged GK5 (isoform 2) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
TRRAP sgRNA2

Plasmid

#138183

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRRAP

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

TRRAP (TRRAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
B-eSpCas9

Plasmid

#126761

Purpose

Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

B-eSpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

K848A; K1003A; R1060A; amino acids 1005-1013 repl…

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
B-evoSpCas9

Plasmid

#126765

Purpose

Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

B-evoSpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

M495V; Y515N; K526E; R661Q; amino acids 1005-1013…

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ETV7_A86D_pet28a

Plasmid

#131657

Purpose

Bacterial expression of ETS gene with A86D mutation for solubility

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV7 A86D (ETV7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Alanine 86 to Aspartic Acid

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pmKO-AF8

Plasmid

#118224

Purpose

Kusabira- Orange-CENP-A expression plasmid

Depositor

Kenji Sugimoto

Article

Sugimoto et al Mutat Res. 2008 Nov 17;657(1):56-62. doi

Insert

CENP-A (CENPA Human)

Tags

Kusabira orange

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PHAGE-P CMVt N-HA hE6-AP III (AA 1-84)

Plasmid

#119319

Purpose

lentiviral vector for expression of HA-Tagged E6-AP III AA 1-84 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP III (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 1-84

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PHAGE-P CMVt N-HA hE6-AP I (AA 1-500)

Plasmid

#119312

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 1-500 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 1-500

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PHAGE-P CMVt N-HA hE6-AP I (AA 1-376)

Plasmid

#119311

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 1-376 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 1-376

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiSAMv2 TCF7L2 TSS-guide4

Plasmid

#118187

Purpose

CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

dCas9-VP64-2A-BlastR

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core and U6

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_hE2F7mg_2ntmut

Plasmid

#73074

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns, 2…

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pET22b-ecDHFR-G51PEKN

Plasmid

#118582

Purpose

Bacterial expression of E.coli DHFR G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Expression

Bacterial

Mutation

G51 mutated to P51; Glu-Lys-Asn residues inserted…

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-shMBNL3-9465

Plasmid

#115464

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL3-9465 (MBNL3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_BRD003-sgRBFOX1-2083

Plasmid

#115451

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgRBFOX1-2083 (RBFOX1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4-Tet-on-vsv-Mis12-dCEN Incenp

Plasmid

#108482

Purpose

inducible expression of vsv-Mis12-dCEN-INCENP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a- mECitrine-kanMX6

Plasmid

#105150

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

mECitrine-kanMX6

Tags

mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Cx32R220stop

Plasmid

#78151

Purpose

Mammalian Expression of the first 219 amino acids of Connexin 32 and EGFP from a bicistronic mRNA

Depositor

Mario Bortolozzi

Article

Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496

Insert

Connexin 32 (GJB1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Only contains first 219 residues

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only
pSN67

Plasmid

#100124

Purpose

expresses putative KLF4 DBD with a 6xHis tag

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCherry-P2A-putative KLF4 DBD with a 6xHis tag (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Insert corresponds to the DBD of KLF4

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSN69

Plasmid

#100125

Purpose

expresses putative cMYC DBD with a HA tag

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCerulean-P2A-putative cMYC DBD with a HA tag (MYC Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Insert corresponds to the DBD of cMYC

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE/SP N184X with 5'UTR

Plasmid

#92375

Purpose

Inducible expression of N184X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

S184X

Promoter

Tet-responsive P tight

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge