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pAC1821_pCR8-SMN2-DN-RG6-SA

Plasmid

#118655

Purpose

Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

SMN2-DN-RG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Filamin-C-stFRET

Plasmid

#61107

Purpose

Expression of Filamin with C-term stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-non-targeting (pJK5)

Plasmid

#229052

Purpose

Cloning backbone for PspCas13b crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pICSL80005

Plasmid

#50322

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, variant of turboGFP codon-optimised for plants (Pontellina plumata)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pSIBR4-Cre

Plasmid

#219435

Purpose

Theophylline_induced self_splicing intron_based riboswitch for the moderate expression of Cre recombinase

Depositor

Sheng Yang

Article

Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

Insert

intron 4-Cre

Expression

Bacterial

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pmU1-CasRx-non-targeting (pKW83)

Plasmid

#229051

Purpose

Cloning backbone for CasRx crRNA (Mouse U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[sfGFP]

Plasmid

#213902

Purpose

Mariner transposon expressing sfGFP with kan selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and superfolder GFP

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pICH71431

Plasmid

#50344

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, ATPase (S. lycopersicum )

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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Filamin-M-stFRET

Plasmid

#61105

Purpose

Expression of Filamin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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Actinin-M-stFRET

Plasmid

#61103

Purpose

Expression of α-actinin with internal stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2015

Availability

Academic Institutions and Nonprofits only

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pGGY001

Plasmid

#48866

Purpose

Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible plant transformation vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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CelTag Plasmid

Plasmid

#66562

Purpose

Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

Depositor

David Engelke

Article

Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

Insert

CelTag

Expression

Bacterial

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pSuper-gmiR-1

Plasmid

#171553

Purpose

a human miR-1-expressing plasmid

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

miR-1-2 (MIR1-2 Human)

Expression

Mammalian

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGGA004

Plasmid

#48815

Purpose

Provides the 35S promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

35S promoter

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site removed by substit…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGF003

Plasmid

#48844

Purpose

Provides a D-alanine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::D-AlanineR::tMAS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-luc-miR-122

Plasmid

#171557

Purpose

a positive control reporter with 3' UTR sequences perfectly complementary to miR-122

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

a sequences perfectly complementary to miR-122

Expression

Mammalian

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pICH41432

Plasmid

#50343

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, ocs (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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3694 pMIG HA-Bax

Plasmid

#8806

Depositor

Stanley Korsmeyer

Article

Cheng et al Science 2003 Jul 25;301(5632):513-7.

Insert

Bax (Bax Mouse)

Use

Retroviral

Tags

GFP and HA

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only
pICH44022

Plasmid

#50330

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, P19 suppressor of gene silencing (Tomato Bushy Stunt Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Actinin-C-stFRET

Plasmid

#61104

Purpose

Expression of α-actinin with C-term stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

α-actinin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge