We narrowed to 27,563 results for: STI;

Showing: 10121 - 10140 of 27563 results

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pET28-At_PRORP1-His

Plasmid

#67869

Purpose

bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

Depositor

Walter Rossmanith

Article

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP1 (PRORP1 Mustard Weed)

Tags

His

Expression

Bacterial

Mutation

Residues 36-572 (see comments)

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 GATA1 K137R

Plasmid

#85694

Purpose

Mammalian expression of mouse GATA1 with K137R mutation

Depositor

Licio Collavin, Giannino Del Sal

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

GATA1 (Gata1 Mouse)

Expression

Mammalian

Mutation

K137R (SUMO-1 attachment site)

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pEGFP-CD86-mApple

Plasmid

#133860

Purpose

Mammalian expression of CD86 N-terminally tagged with EGFP and C-terminally tagged with mApple

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

CD86 (CD86 Human)

Tags

EGFP and mApple

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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p6900 PHAGE-P CMVT N-HA-hE6AP III C840A

Plasmid

#37606

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP III C840A (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C840A

Promoter

CMV tet regulatable

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
pHelA12

Plasmid

#176493

Purpose

Expresses the N-terminal His6-tagged SARS-CoV helicase in E coli.

Depositor

Xiaodong Cheng, Jian-Dong Huang

Article

Tanner et al J Biol Chem. 2003 Oct 10;278(41):39578-8

Insert

SARS-CoV NSP13 (ORF1ab SARS-CoV)

Tags

His6 and T7

Expression

Bacterial

Mutation

V570I

Promoter

T7

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant

Plasmid

#101202

Purpose

Bacterial expression plasmid for SpCas9∆REC3 variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant M1–N497,GGS,V713–D1368

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

M1–N497, GGS, V713–D1368

Promoter

T7

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS2131

Plasmid

#111895

Purpose

ssAAV genome with MCS for inserting MiniP promoter to drive an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Type

Empty backbone

Use

AAV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pPN461

Plasmid

#137877

Purpose

PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

POGZ (POGZ Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS5-Af-mut

Plasmid

#120829

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

ZIKV NS5 V114M/V872M - "african mut"

Tags

V5

Expression

Mammalian

Mutation

V114M/V872M

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL3-2

Plasmid

#109010

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #2 (ATL3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS1964

Plasmid

#82574

Purpose

High copy number plasmid containing Ple305 MiniPromoter insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple305

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 23, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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KN302: pMVP/SB/eCFP-DEST

Plasmid

#121863

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KK901: pMVP/SB/Zeo-DEST

Plasmid

#121868

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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R4pGWB401+Citrine

Plasmid

#128432

Purpose

Modified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.

Depositor

George Haughn

Article

McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

Type

Empty backbone

Use

Gateway destination vector

Tags

Citrine, yellow fluorescence protein

Expression

Plant

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-2TAG

Plasmid

#158138

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the first serine residue…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-His-myc-Uba1

Plasmid

#29508

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Uba1 (UBA1 Budding Yeast)

Tags

His and myc

Expression

Insect

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR sgHAL

Plasmid

#102315

Purpose

genetic depletion of HAL

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgHAL (HAL Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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px330 CTCF gRNA

Plasmid

#165588

Purpose

Insertion of CTCF degon

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

CTCF (CTCF Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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px330 CBP gRNA

Plasmid

#165587

Purpose

insertin of CBP degrom

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

CBP (Crebbp Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pHL 1917

Plasmid

#53024

Purpose

AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Inserts

mCherry
fimB (fimB E. coli)

Use

Synthetic Biology

Tags

Asp terminator, RBS(st7), and T1 terminator

Expression

Bacterial

Promoter

pCon and pLlacO-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only