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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,050 results for: nin

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Showing: 10121 - 10140 of 66050 results

  2. pKK-MBP-TEV

    Plasmid
    #105771
    Purpose
    Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    MBP-TEV
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105771-plasmid-map-sequence-id-203360

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  12. pCasso

    Plasmid
    #207530
    Purpose
    Conditionally-replicating in Pseudomonas plasmid for cytidine base editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sg
    Depositor
    Pablo Ivan Nikel
    Publication
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    cytidine base editing; oriV(pRO1600/ColE1), xylS, Pm→repA, P14b→msfGFP; Pm→CBE:SpRY, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Available since
    Feb. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    207530-plasmid-map-sequence-id-422503

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  15. pEVOL-NBK-1

    Plasmid
    #207639
    Purpose
    A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.
    Depositor
    Cole DeForest
    Publication
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
    Expression
    Bacterial
    Mutation
    The evolved synthetase NBK-1 contains mutations Y…
    Promoter
    araBAD
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207639-plasmid-map-sequence-id-409890

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  16. pJAT74

    Plasmid
    #204296
    Purpose
    pJAT plasmid with multiple cloning site for introducing custom payload. One U6 promoter.
    Depositor
    David Stern
    Publication
    Stern et al bioRxiv 2023.06.17.545412
    Type
    Empty backbone
    Use
    CRISPR
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204296-plasmid-map-sequence-id-406186

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  17. pSL5069 (pDonor(AAVS1),CRISPR_Pse)

    Plasmid
    #200904
    Purpose
    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.
    Depositor
    Samuel H. Sternberg
    Publication
    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103
    Insert
    Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
    Expression
    Mammalian
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200904-plasmid-map-sequence-id-393155

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Showing: 10121 - 10140 of 66050 results