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pET-24a-ELP[V-30]

Plasmid

#67014

Purpose

Expresses ELP[V-30] in E. coli

Depositor

Ashutosh Chilkoti

Publication

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

ELP[V-30]

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1_PsChR2_EYFP

Plasmid

#69057

Purpose

High-efficiency blue-shifted cation channelrhodopsin

Depositor

John Spudich

Publication

Govorunova et al J Biol Chem. 2013 Oct 11;288(41):29911-2

Insert

PsChR2

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET11a-link-NGFP

Plasmid

#52732

Purpose

IPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Publication

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

link-NGFP

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET His6 ProteinG TEV LIC cloning vector (2P-T)

Plasmid

#29713

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-ProteinG-TEV

Expression

Bacterial

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFLAG-6a-Ataxin3Q22

Plasmid

#22126

Depositor

Henry Paulson

Insert

Ataxin 3 (ATXN3 Human)

Tags

Flag

Expression

Mammalian

Available since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pRU1097

Plasmid

#14462

Depositor

Philip Poole

Publication

Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

Insert

gfp-mut3.1

Expression

Bacterial

Available since

April 23, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-CAG-Cre

Plasmid

#196140

Purpose

AAV-mediated expression of Cre under the CAG promoter .

Depositor

Edward Boyden

Publication

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Cre

Use

AAV

Expression

Mammalian

Mutation

codon diversified

Promoter

CAG

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PGK-DTAG-NTERM-GFP

Plasmid

#185775

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Publication

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, dTAG

Mutation

none

Promoter

PGK

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJRH051

Plasmid

#171625

Purpose

2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

Depositor

Jennifer Doudna

Publication

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-BsmBI-Destination
mNeon

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1-a and U6

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBabe_Hyg_cOVA_T2A_mStrawberry

Plasmid

#161737

Purpose

Expression of cOVA and mStrawberry

Depositor

Alec Kimmelman

Publication

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

cytoplasmic ovalbumin

Use

Retroviral

Tags

mStrawberry

Expression

Mammalian

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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LT3GECIR

Plasmid

#111178

Purpose

Tet-ON miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Publication

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Mutation

WT

Promoter

T3G

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSMP-Dot1L_1

Plasmid

#36339

Depositor

George Daley

Publication

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Dot1L (DOT1L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEGFP-C1-TFIIB

Plasmid

#26675

Depositor

Sui Huang

Publication

Chen et al Mol Biol Cell. 2002 Jan . 13(1):276-84.

Insert

TFIIB (GTF2B Human)

Tags

EGFP

Expression

Mammalian

Available since

Nov. 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCAG-hHDAC6

Plasmid

#21099

Depositor

Connie Cepko

Publication

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

Histone Deacetylase 6 (HDAC6 Human)

Expression

Mammalian

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
RCAS-myc

Plasmid

#11548

Depositor

Sandra Orsulic

Publication

Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

Insert

c-myc (MYC Human)

Use

Retroviral

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
fluoPEER-GFP>BFP

Plasmid

#185477

Purpose

Fluorescent reporter for a Y66H mutation to convert a GFP to BFP.

Depositor

Sabine Fuchs

Publication

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

GFP-Y66-editing-site

Expression

Mammalian

Promoter

CMV

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV-egfp-U3-Osp.gRNA

Plasmid

#166034

Purpose

Empty backbone for expression of gRNA-expressing integration deficient lentiviral particles.

Depositor

Yujia Cai

Publication

Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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Myc-Bnip3FL

Plasmid

#100796

Purpose

Mammalian expression of Bnip3 full length splice variant

Depositor

Joseph Gordon

Publication

Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

Insert

mus Bnip3 (Bnip3 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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miReporter-PGK

Plasmid

#82477

Purpose

microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

Insert

H2B-mCherry and H2B-Citrine. HygroR

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pE2-Orange-N1

Plasmid

#21719

Depositor

Benjamin Glick

Publication

Strack et al BMC Biotechnol. 2009 Apr 3;9:32. doi: 10

Insert

E2-Orange

Expression

Mammalian

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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