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Showing: 2501 - 2550 of 8038 results

  9. pENT-L3-pSnail-L5

    Plasmid
    #186365
    Purpose
    Entry clone with ORF encoding Ci-Snail cis-regulatory region flanked by Gateway recombination sequences
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    Snail regulatory sequence
    Use
    Protein expression
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter
    Available since
    Nov. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    186365-plasmid-map-sequence-id-373437

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  10. pENT-R4-bpFOG-L5

    Plasmid
    #186369
    Purpose
    Entry clone with ORF encoding Strong basal promoter of FOG flanked by Gateway recombination sequences
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    Basal promoter of the Friend Of Gata gene
    Use
    Protein expression
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    186369-plasmid-map-sequence-id-373448

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  11. pENT-L3-pFOGl-L5

    Plasmid
    #186364
    Purpose
    Entry clone with ORF encoding Ci-FOG cis-regulatory region flanked by Gateway recombination sequences
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    Friend Of Gata regulatory sequence
    Use
    Protein expression
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    186364-plasmid-map-sequence-id-361706

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  12. pENT-L3-a-element-L4

    Plasmid
    #186366
    Purpose
    Entry clone with ORF encoding Otx cis-regulatory region flanked by Gateway recombination sequences
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    a-element early Otx neural enhancer
    Use
    Protein expression
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186366-plasmid-map-sequence-id-373439

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  19. pME-GalT

    Plasmid
    #109558
    Purpose
    Multisite gateway vector for 5' tagging with the N-terminal sequence from zebrafish 1,4 galactosyltransferase 1 (GalT). Marks the Golgi complex.
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    the N-terminal sequence from zebrafish 1,4 galactosyltransferase 1 (GalT)
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109558-plasmid-map-sequence-id-223872

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  23. pENT-L1-Kozak-HistoneH2B-L2

    Plasmid
    #186357
    Purpose
    Entry clone with ORF encoding histone H2B flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    Histone H2B (H2BC11 Human)
    Use
    Expression of a tagged nuclear marker
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186357-plasmid-map-sequence-id-373425

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  24. pME-mKate2-GPI-pA

    Plasmid
    #109542
    Purpose
    Multisite gateway vector for expression of mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109542-plasmid-map-sequence-id-223866

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  26. pME-mKate2-KDEL-pA

    Plasmid
    #109546
    Purpose
    Multisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109546-plasmid-map-sequence-id-223868

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  30. pME-H2B

    Plasmid
    #108883
    Purpose
    Multisite gateway vector for 5' tagging with the human histone H2B sequence (for nuclear localisation)
    Depositor
    Rob Parton
    Article
    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do
    Insert
    H2B (human amino acid sequence but different codon usage) (H2BC21 Synthetic, Human)
    Use
    Multisite gateway
    Tags
    Expression
    Mutation
    human amino acid sequence but different codon usa…
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    108883-plasmid-map-sequence-id-210105

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  32. pENT-L1-Kozak-Ark-L2

    Plasmid
    #186362
    Purpose
    Entry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    Ciona robusta Aurora kinase A
    Use
    Expression of a fluorescent centrosome marker
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    186362-plasmid-map-sequence-id-373433

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  33. pRSM4

    Plasmid
    #101642
    Purpose
    Encodes the fluorescent protein KCyG4219 for constitutive expression in E. coli.
    Depositor
    Thomas Hughes
    Article
    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548
    Insert
    KCyG4219
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    C-terminal residues (221-223) deletion. Ser220Val…
    Promoter
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    101642-plasmid-map-sequence-id-195172

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  37. pcDNA3-sPDGFRalpha-Fc

    Plasmid
    #136438
    Purpose
    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Insert
    PDGFRalpha (PDGFRA Human)
    Use
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…
    Expression
    Mammalian
    Mutation
    Extracellular domain of PDGFR-alpha (a.a. 1-524)
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136438-plasmid-map-sequence-id-271697

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  38. pCI-Flag-NEDD4L∆WW1,2,4

    Plasmid
    #171137
    Purpose
    expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells
    Depositor
    Wesley Sundquist
    Article
    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10
    Insert
    NEDD4L (NEDD4L Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed tryptophan 221 to alanine, tryptophan 453…
    Promoter
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171137-plasmid-map-sequence-id-338242

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  39. pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2

    Plasmid
    #186361
    Purpose
    Entry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    Human Ensconsin (MAP7 Synthetic, Human)
    Use
    Expression of a fluorescent microtubule marker
    Tags
    3xEGFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186361-plasmid-map-sequence-id-373431

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  48. TFORF0891

    Plasmid
    #142211
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ING4 (ING4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142211-plasmid-map-sequence-id-397720

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  49. LV-AR-R599A-N600A

    Plasmid
    #171221
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: R599A-N600A, mutations in the D-box of the Zinc finger 2 that mediates AR dimerization.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-R599A-N600A (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-R599A-N600A
    Promoter
    EF-1a
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171221-plasmid-map-sequence-id-342450

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  50. pET His6 MBP TEV HDAC8_374

    Plasmid
    #122174
    Purpose
    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
    Depositor
    David Christianson
    Article
    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22
    Insert
    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    Use
    Tags
    hexahistidine tag and tobacco etch virus protease…
    Expression
    Bacterial
    Mutation
    Residues Met1–Pro7 and H375-V377 have been delete…
    Promoter
    T7
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122174-plasmid-map-sequence-id-237469

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Showing: 2501 - 2550 of 8038 results