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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,101 results for: CAG

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Showing: 1001 - 1020 of 10101 results

  1. AAV-CAG-FLEX-NES-jGCaMP8f-WPRE

    Plasmid
    #186044
    Purpose
    AAV-mediated cytosolic expression of jGCaMP8f under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    NES-jGCaMP8f
    Use
    AAV
    Tags
    NES-6xHis
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186044-plasmid-map-sequence-id-370163

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  2. pSLQ10873

    Plasmid
    #183961
    Purpose
    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR
    Depositor
    Stanley Qi
    Publication
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    183961-plasmid-map-sequence-id-369669

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  3. Target-AID (pRZ762)

    Plasmid
    #131300
    Purpose
    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131300-plasmid-map-sequence-id-254651

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  4. H3K9me3 biosensor

    Plasmid
    #120802
    Purpose
    FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET
    Depositor
    Yingxiao Wang
    Publication
    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120802-plasmid-map-sequence-id-235185

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  8. pCAG-mCherry2-ER

    Plasmid
    #164166
    Purpose
    Expresses mCherry2 fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter
    Depositor
    Isei Tanida
    Publication
    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry2
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Feb. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    164166-plasmid-map-sequence-id-323961

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  10. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid
    #121171
    Purpose
    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    xFNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121171-plasmid-map-sequence-id-235381

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  11. M-NM-sgRNA

    Plasmid
    #48673
    Purpose
    Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter
    Use
    CRISPR
    Promoter
    hU6
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48673-plasmid-map-sequence-id-122901

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  12. pCAG_EGFP-RandE3

    Plasmid
    #79870
    Purpose
    Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.
    Depositor
    Don Arnold
    Publication
    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP-RandE3
    Use
    Synthetic Biology
    Tags
    GFP and HA
    Expression
    Mammalian
    Promoter
    Beta-actin
    Available since
    Aug. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    79870-plasmid-map-sequence-id-149706

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  13. M-ST1-sgRNA

    Plasmid
    #48672
    Purpose
    Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter
    Use
    CRISPR
    Promoter
    hU
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48672-plasmid-map-sequence-id-71846

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  14. AAV-CAG-M-red-SPOTIT2

    Plasmid
    #191491
    Purpose
    Red fluorescent-based opioid sensor for the mu opioid receptor in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    M-red-SPOTIT2
    Use
    AAV
    Promoter
    CAG
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191491-plasmid-map-sequence-id-377934

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  18. PB CAG-F-bpA-F-L-td-sfGFP

    Plasmid
    #133580
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Expression
    Mammalian
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133580-plasmid-map-sequence-id-274421

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  19. TLCV2-RB1

    Plasmid
    #87836
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.
    Depositor
    Adam Karpf
    Publication
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Insert
    sgRB1 (RB1 Human)
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Expression
    Mammalian
    Promoter
    Tight TRE promoter and U6
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87836-plasmid-map-sequence-id-176310

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  20. AAV-CAG-jGCaMP8f-WPRE (AAV9)

    Viral prep
    #179254-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-CAG-jGCaMP8f-WPRE (#179254). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8f-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    CAG
    Available since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 1001 - 1020 of 10101 results