We narrowed to 4,910 results for: D10

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pEF-AncBE4max

Plasmid

#138270

Purpose

Expression of AncBE4max-Cas9 Base-editor from an EF1a promoter.

Depositor

Xiao Wang

Publication

Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AncBE4max

Use

CRISPR

Expression

Mammalian

Mutation

D10A

Promoter

Ef1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pYPQ151

Plasmid

#69302

Purpose

Gateway entry vector with pcoCas9D10A

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pcoCas9D10A (Plant codon-optimized)

Use

CRISPR

Tags

2XFLAG

Mutation

D10A mutation; NLS fusion

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2FE-ABE8e-SpRY

Plasmid

#213008

Purpose

A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

Depositor

Richard Sherwood

Publication

Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

Insert

ABE8e-SpRY-D10A

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A nickase variant of SpRY

Promoter

Ef-1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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CBE-T1.52

Plasmid

#193285

Purpose

Expression of CBE-T1.52 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Publication

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CBE-T1.52

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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YEE-BE4max

Plasmid

#138157

Purpose

C-to-T base editor

Depositor

David Liu

Publication

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rAPOBEC1(YEE)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDicAID_nCas9-PmCDA-2A-NptII_ETR

Plasmid

#91695

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

Depositor

Akihiko Kondo

Publication

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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lentiCRISPRv2FE-ABE8e-SpRY

Plasmid

#213008

Purpose

A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

Depositor

Richard Sherwood

Publication

Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

Insert

ABE8e-SpRY-D10A

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A nickase variant of SpRY

Promoter

Ef-1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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CBE-T1.52

Plasmid

#193285

Purpose

Expression of CBE-T1.52 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Publication

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CBE-T1.52

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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ABE8.20-d

Plasmid

#136301

Purpose

expresses ABE8.20-d in mammalian cells

Depositor

Nicole Gaudelli

Publication

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABE8.20-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Promoter

CMV

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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YEE-BE4max

Plasmid

#138157

Purpose

C-to-T base editor

Depositor

David Liu

Publication

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rAPOBEC1(YEE)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E + R132E; within Cas9…

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDicAID_nCas9-PmCDA-2A-NptII_ETR

Plasmid

#91695

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

Depositor

Akihiko Kondo

Publication

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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ABE8.20-d

Plasmid

#136301

Purpose

expresses ABE8.20-d in mammalian cells

Depositor

Nicole Gaudelli

Publication

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABE8.20-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Promoter

CMV

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBECKP-km

Plasmid

#117235

Purpose

A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Publication

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAC2-dual-dCas9VP48-sgExpression

Plasmid

#48236

Purpose

Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9VP48

Use

CRISPR

Tags

HA-tag and VP48

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBECKP-km

Plasmid

#117235

Purpose

A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Publication

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAC2-dual-dCas9VP48-sgExpression

Plasmid

#48236

Purpose

Dual expression construct expressing both dCas9VP48 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9VP48

Use

CRISPR

Tags

HA-tag and VP48

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV_ABEmax_3xHA

Plasmid

#112098

Purpose

A:T-to-G:C base editing

Depositor

David Liu

Publication

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABEmax_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEF_dCas9

Plasmid

#68416

Purpose

Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A/H841A

Promoter

human EF1[alpha]

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pRH2502

Plasmid

#84379

Purpose

Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

Depositor

Robert Husson

Publication

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

dcas9

Use

CRISPR

Expression

Bacterial

Mutation

Aspartate at position 10 changed to Alanine, Hist…

Promoter

uv15tetO

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMV_ABEmax_3xHA

Plasmid

#112098

Purpose

A:T-to-G:C base editing

Depositor

David Liu

Publication

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABEmax_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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