Addgene

We narrowed to 7,585 results for: Lif;

Showing: 1001 - 1020 of 7585 results

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hygII-UT-ABAleon2.15

Plasmid

#106983

Purpose

ABAleon2.15 in plant expression vector (Hygromycin resistance)

Depositor

Julian Schroeder

Article

Waadt et al Elife. 2014 Apr 15;3:e01739. doi: 10.755

Insert

ABAleon2.1

Use

Tags

Expression

Plant

Mutation

Promoter

UBQ10

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCO487

Plasmid

#139730

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, H3H

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_B_AtUbi10:NnH3H:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

CmYLCV

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac211-RPL13A-FKBPx2

Plasmid

#104473

Purpose

Plasmid for pop-in/pop-out replacement of RPL13A with an FKBPx2-tagged version.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

RPL13Ap (RPL13A Budding Yeast)

Use

Tags

FKBPx2-HA

Expression

Yeast

Mutation

includes the last 354 bp of RPL13A only

Promoter

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCO488

Plasmid

#139731

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Hisps

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_C'_CmYLCV:NnHisps:tHsp

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

CmYLCV

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pQIq_MRGS_HIS8_( E3_5)_FLAG

Plasmid

#199612

Purpose

Encodes DARPin-FLAG E3_5 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG E3_5 (control)

Use

Tags

8xHis and FLAG

Expression

Bacterial

Mutation

Promoter

T5

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-Gbait-hs-QF2

Plasmid

#122563

Purpose

Integration of QF2 sequence using the CRISPR/Cas9 system.

Depositor

Marnie Halpern

Article

Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

Insert

Gbait-hsp70-QF2-SV40pA

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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NK66

Plasmid

#176606

Purpose

To measure Syk intensity in response to ligand clustering using DNA origami

Depositor

Meghan Morrissey

Article

Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

Syk-BFP (Syk Mouse, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

n/a

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pF(UG) CamKII sf iGluSnFR

Plasmid

#158769

Purpose

iGluSnFR glutamate sensor containing a Golgi export sequence and and an ER exit motif for improved trafficking to the plasma membrane.

Depositor

Edwin Chapman

Article

Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

iGluSnFR

Use

Lentiviral

Tags

Expression

Mutation

Additional ER and Golgi export sequences

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGM-ENO2-mCherry-YFP

Plasmid

#180570

Purpose

Dual fluorescent reporter for 5' UTR activity in yeast mCherry/YFP

Depositor

Joel McManus

Article

May et al Elife. 2023 May 25;12:e69611. doi: 10.75

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGM-ENO2-YFP-mCherry

Plasmid

#180569

Purpose

Dual fluorescent reporter for 5' UTR activity in yeast YFP/mCherry

Depositor

Joel McManus

Article

May et al Elife. 2023 May 25;12:e69611. doi: 10.75

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pT3TS-NLS-TurboID

Plasmid

#163849

Purpose

In vitro transcription of nls-TurboID. Parton lab clone JST

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

In vitro transcription of rna

Tags

nls

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescriptKS(-)-FRBx2

Plasmid

#104476

Purpose

Plasmid carrying FRBx2 with C-terminal HA tag.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

FRBx2 (MTOR Human)

Use

Phagemid

Tags

HA tag

Expression

Mutation

includes only the FRB domain

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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P442

Plasmid

#139728

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz, promoter is ABA responsive

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_D_pAtRAB18:NnLuz:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

AtRAB18

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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P223

Plasmid

#139720

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, CPH

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_A'_pLHB1B1:NnCPH:tSlH4

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

AtLHB1B1

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pGEM-T Easy-Gbait-hs-Cre

Plasmid

#122562

Purpose

Integration of Cre sequence using the CRISPR/Cas9 system.

Depositor

Marnie Halpern

Article

Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

Insert

Gbait-hsp70-Cre-SV40pA

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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P0050::rpaA (KmR)

Plasmid

#102321

Purpose

drives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with p0050 promoter (+ kanamycin resistance casette)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

-

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pME-miniTurbo

Plasmid

#166567

Purpose

Multisite Gateway middle entry clone encoding miniTurbo. Parton lab clone JRQ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pME-BASU-NS

Plasmid

#166565

Purpose

Multisite Gateway middle entry clone encoding BASU. Parton lab clone JRO

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

BASU

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFLIP38

Plasmid

#65616

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Use

Tags

6His-edCit-attR1 and attR2-edCer-cMyc

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pFLIP43

Plasmid

#65618

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Use

Tags

6His-edAFPt9-attR1 and attR2-edCer-cMyc

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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