We narrowed to 15,654 results for: NOL

1. pNCS tdTomato

   Plasmid

   #91767

   Purpose

   Bacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato)

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Insert

   tandem dimer Tomato
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUW-tetO-Stat5

   Plasmid

   #139832

   Purpose

   doxycycline-inducible expression of mouse Stat5 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Stat5 (Stat5a Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE-mCMV

   Available Since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LZF97_hSyn-spine-jRGECO1a

   Plasmid

   #119198

   Purpose

   Targets jRGECO1a to spines in living neurons.

   Depositor

   Don Arnold

   Article

   Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

   Insert

   jRGECO1a-TPR3-Utrophin(1-78)-TPR3-Zfn(CCR5-L) (Utrn Synthetic, Rat)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Human Synapsin 1

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHLuc Control

   Plasmid

   #141091

   Purpose

   pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUW-tetO-NFE2

   Plasmid

   #139825

   Purpose

   doxycycline-inducible expression of Human NFE2 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   NFE2 (NFE2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE-mCMV

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUW-tetO-Nfil3

   Plasmid

   #139815

   Purpose

   doxycycline-inducible expression of mouse Nfil3 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Nfil3 (Nfil3 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE-mCMV

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG_EGFP-RandE3

   Plasmid

   #79870

   Purpose

   Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Promoter

   Beta-actin

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMA_Int

   Plasmid

   #110096

   Purpose

   E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Insert

   mycophage integrase
   

   Use

   Required for transformation of attb integrating v…

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACEC3GH (Apr)

   Plasmid

   #110085

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pExpreS2-1-C3-PA

    Plasmid

    #175446

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Tags

    3C-Protein A and BiP Secretion Peptide

    Expression

    Insect

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUW-tetO-Zbtb46

    Plasmid

    #139811

    Purpose

    doxycycline-inducible expression of mouse Zbtb46 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Zbtb46 (Zbtb46 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. AQTFAATHNPWASQA_epitope

    Plasmid

    #165019

    Purpose

    Expression of CMV UL86 (1222-1236) fused to CD74

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    CMV UL86 (1222-1236)
    

    Use

    Lentiviral

    Tags

    CD74 at N-term; cathepsin S cleavage site (GRWHTV…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a Gx-NL2

    Plasmid

    #169107

    Purpose

    Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc
    

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF1a-T2-NLuc

    Plasmid

    #135938

    Purpose

    Expresses NanoLuciferase targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MPC11 IgG2b gene

    Plasmid

    #127248

    Purpose

    Expresses intact IgG2b gene in mammalian cells

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    IgG2b gene with exons and alt pAs (Ighg2b Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTJK512

    Plasmid

    #121469

    Purpose

    CEN LEU2 CNB1

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNB1 (CNB1 Budding Yeast)
    

    Use

    Yeast cen plasmid

    Promoter

    Endogenous genomic promoter region

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-mPar6a WT

    Plasmid

    #86148

    Purpose

    FLAG tagged mouse Par6 wild type - Binds to aPKC, Par3, Lgl, Cdc42

    Depositor

    Senthil Muthuswamy

    Article

    Nolan et al Cancer Res. 2008 Oct 15;68(20):8201-9.

    Insert

    Par6 (Pard6a Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pGB1-mCRT(P-domain)

    Plasmid

    #83519

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

    Insert

    CALR (Calr Mouse)
    

    Tags

    GB1 and His

    Expression

    Bacterial

    Mutation

    P domain (contains residues 187-283)

    Promoter

    T7

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pGB1-mCRT(C-domain)

    Plasmid

    #83518

    Purpose

    Bacterial expression of Calreticulin mutant

    Depositor

    Malini Raghavan

    Article

    Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

    Insert

    CALR (Calr Mouse)
    

    Tags

    GB1 and His

    Expression

    Bacterial

    Mutation

    C domain (contains residues 340-399)

    Promoter

    T7

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pTRE3G-NS3/4A

    Plasmid

    #186884

    Purpose

    Doxycycline-dependent expression of NS3/4A protease gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    HCV NS3/4A protease
    

    Use

    Retroviral

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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