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TALE-free DddA6-11 1397C

Plasmid

#221943

Purpose

Expresses mitochondrially targeted TALE-free DddA6/11 1397C-UGI in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-DddA6/11 1397C-UGI-ATP5B 3'UTR

Tags

3xFLAG

Expression

Mammalian

Mutation

DddA: T1413I

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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TALE-free DddA6 1397N

Plasmid

#221944

Purpose

Expresses mitochondrially targeted TALE-free DddA6 1397N-UGI in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-DddA6 1397N-UGI-ATP5B 3'UTR

Tags

3xFLAG

Expression

Mammalian

Mutation

DddA: Q1310R, S1330I, T1380I

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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TALE-free DddA11 1397N

Plasmid

#221945

Purpose

Expresses mitochondrially targeted TALE-free DddA11 1397N-UGI in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-DddA11 1397N-UGI-ATP5B 3'UTR

Tags

3xFLAG

Expression

Mammalian

Mutation

DddA: S1330I, A1341V, N1342S, E1370K, T1380I

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-C-GFP-NLS

Plasmid

#222604

Purpose

Expression of MAC3-tagged GFP-NLS (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 5' nuclear localizaition sequence (NLS) (PPKTTRKVED)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-N-GFP-NES

Plasmid

#222606

Purpose

Expression of MAC3-tagged GFP-NES (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 3' nuclear exclusion sequence (NES)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-NTRK3

Plasmid

#222612

Purpose

Expression of MAC3-tagged NTRK3

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

NTRK3 (NTRK3 Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-N-GFP-NLS

Plasmid

#222607

Purpose

Expression of MAC3-tagged GFP-NLS (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 3' nuclear localizaition sequence (NLS)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-C-GFP-NES

Plasmid

#222603

Purpose

Expression of MAC3-tagged GFP-NES (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 5' nuclear exclusion sequence (NES)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-N-GFP-Myr

Plasmid

#222608

Purpose

Expression of MAC3-tagged GFP-Myr (N-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 3' myristoylation sequence (Myr)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-C-GFP-Myr

Plasmid

#222605

Purpose

Expression of MAC3-tagged GFP-Myr (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 5' myristoylation sequence (Myr)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBi2FC-N-MPK8-P2A:mCherry

Plasmid

#216138

Purpose

Controls for Bicistronic BiFC

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Insert

MPK8

Expression

Plant

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBi2FC-C-MGGAGM-P2A:mTagBFP2

Plasmid

#216141

Purpose

Vector control for Bicistronic BiFC

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Insert

MGGAGM Linker

Expression

Plant

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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msFam160b1 (msFHIP2A) g1 lentiCRISPRv2-mCherry

Plasmid

#218654

Purpose

Knockout vector for mouse Fam160b1 (Fhip2A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Fam160b1 (Fhip2a Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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msFam160b1 (msFHIP2A) g2 lentiCRISPRv2 mCherry

Plasmid

#218655

Purpose

Knockout vector for mouse Fam160b1 (Fhip2A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

Fam160b1 (Fhip2a Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

Plasmid

#218636

Purpose

Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

H781E, Q782R, F785A

Promoter

UBC

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)

Plasmid

#218635

Purpose

Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

N1008R, R1009E, E1010R, R1013E

Promoter

UBC

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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GOLIM4 g2 LentiCRISPRv2-mCherry

Plasmid

#218663

Purpose

Knockout vector for human GOLIM4 (GPP130/GOLPH4)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

GOLIM4 (GOLIM4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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GOLIM4 g1 LentiCRISPRv2-mCherry

Plasmid

#218662

Purpose

Knockout vector for human GOLIM4 (GPP130/GOLPH4)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

GOLIM4 (GOLIM4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB11785

Plasmid

#212699

Purpose

gRNA targeting to the intergradtion site E4

Depositor

Irina Borodina

Article

Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

Insert

gRNA targeting to the intergradtion site E4

Expression

Yeast

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template

Plasmid

#215000

Purpose

Endogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template (Tmem115 Mouse)

Use

Hdr template

Tags

3xHA

Promoter

None

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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