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pICH87633

Plasmid

#50271

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, nos, (Agrobacterium tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pX165-xCas9

Plasmid

#140568

Purpose

Expresses xCas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

A262T R324L S409I E480K E543D M694I E1219V

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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Golgi-DKAR

Plasmid

#49790

Purpose

FRET-based, Golgi-targeted kinase activity reporter for PKD

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2010 Jul 23;285(30):22748-5

Insert

D kinase activity reporter

Tags

CFP, YFP, and eNOS(1-33)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
hGFAP-Roxed-Cre

Plasmid

#51263

Purpose

Expression of the Dre-dependent Roxed-Cre gene under the control of the human GFAP promoter

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

Roxed-Cre

Promoter

hGFAP

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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orthodenticle

Plasmid

#37285

Depositor

Norbert Perrimon

Article

Finkelstein et al Genes Dev. 1990 Sep;4(9):1516-27.

Insert

orthodenticle (oc Fly)

Expression

Bacterial

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
MSX1-pET

Plasmid

#21024

Depositor

Corey Largman

Article

Stelnicki et al Differentiation. 1997 Oct . 62(1):33-41.

Insert

MSX1 (Msx1 Mouse)

Tags

His and T7 tag

Expression

Bacterial

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pSEM236 - pEXP[Pmlc-2 | histamine | rpl-3 UTR]

Plasmid

#159797

Purpose

Histamine based negative selection marker to paralyze animals with arrays

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

Insert

pEXP[Pmlc-2 | histamine | rpl-3 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-H2B-mCherry

Plasmid

#135604

Purpose

Expression of H2B-mCherry mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

H2B-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Promoter

Unknown

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pWZL Hygro- TRF2 deltaB deltaM

Plasmid

#18013

Depositor

Titia de Lange

Article

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pENTR2b-mVenus

Plasmid

#154898

Purpose

mVenus entry vector

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Type

Empty backbone

Use

Entry vector

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLpC-L-IFP-F[1] (mammalian)

Plasmid

#52901

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[2] (mammalian)

Plasmid

#52902

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pSBbi-TIR1/Pur

Plasmid

#171682

Purpose

constitutive expression of TIR1-myc in sleeping beauty transposon vector with selection marker (puromycin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Insert

OsTIR1-MYC

Tags

MYC

Expression

Mammalian

Promoter

EF-1a promoter

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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LI F-G Kan

Plasmid

#104523

Purpose

LI resistance gene module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Kanamycin resistance gene

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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Degron-KI ezh2 donor vector

Plasmid

#65620

Purpose

Donor vector to generate Degron-KI DD-EZH2

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

EZH2 left and right HR arms

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only
pALPS puro miR30-STAT1

Plasmid

#117160

Purpose

Target site ATATCCAGTTCCTTTAGGGCC

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL1P6_crU626

Plasmid

#191781

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P3_crU626

Plasmid

#191778

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P4_crU626

Plasmid

#191779

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P5_crU626

Plasmid

#191780

Purpose

Cas12a guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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