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GFP-ataxin-3 C-t

Plasmid

#89981

Purpose

Expresses the GFP-tagged C-terminus of ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-182

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-XPLN aa452-526

Plasmid

#73381

Purpose

Expresses amino acids 452-526 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 452-526 of XPLN (arhgef3) p…

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa304-466 (PH)

Plasmid

#73380

Purpose

Expresses amino acids 304-466 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 304-466 of XPLN (arhgef3) p…

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa107-311

Plasmid

#73379

Purpose

Expresses amino acids 107-317 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 107-317 of XPLN (arhgef3) p…

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
HA-mRIPK3 T231A/S232A-Flag/cHA-pTRIPZ vector

Plasmid

#73707

Purpose

lentiviral expression of mRIPK3 mutant

Depositor

Francis Chan

Insert

RIPK3 (Ripk3 Mouse)

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

T231A/S232A

Promoter

CMV/tet

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL3

Plasmid

#67418

Purpose

Expression of hArl3 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL6-no stop

Plasmid

#67329

Purpose

hArl6 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL8A-no stop

Plasmid

#67333

Purpose

hArl8a without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-SARA

Plasmid

#67346

Purpose

hSara with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARF5

Plasmid

#67312

Purpose

hArf5 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 477 T to C; silent mutation

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL2-no stop

Plasmid

#67319

Purpose

hArl2 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pONL-N1_Golgi

Plasmid

#65695

Purpose

Expresses Golgi-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

N-terminal 81 amino acids of beta 1,4-galactosyltransferase

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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mKO2-Farnesyl-5

Plasmid

#57904

Purpose

Depositor

Michael Davidson

Insert

Farnesyl (HRAS Human)

Tags

mKO2

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only
pERB161

Plasmid

#58286

Purpose

const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-584 (MID)

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-GFP-FKBPx3
inducible FRB-mCh-Mad1^485-584
FKBP-targeted miRNA

Tags

GFP and mCherry

Expression

Mammalian

Mutation

amino acids 485-584 of Mad1

Available Since

Feb. 19, 2015

Availability

Academic Institutions and Nonprofits only

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mEos2-ALK5-N-13

Plasmid

#57343

Purpose

Localization: Membrane Receptor, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

ALK5 (TGFBR1 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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tdEos-H2B-C-22

Plasmid

#57623

Depositor

Michael Davidson

Insert

H2B22 (H2BC11 Human)

Tags

tdEos

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pRevLCav2

Plasmid

#60806

Purpose

used to make mutations in yeast REV3

Depositor

Thomas Kunkel, Youri Pavlov

Article

Pavlov et al Genetics. 2001 Sep;159(1):47-64.

Insert

REV3 (REV3 Budding Yeast)

Expression

Yeast

Mutation

contains 647 C-terminal amino acids and 335 bp of…

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only

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tdEos-MAPTau-C-10

Plasmid

#57636

Purpose

Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

MAPTau (MAPT Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Xp75 DDD

Plasmid

#61282

Purpose

Expresses Xenopus p75 DDD domain

Depositor

Mark Bothwell, Lara Hutson

Article

Hutson et al J Neurobiol. 2001 Nov 5;49(2):79-98.

Insert

p75 DDD (p75NTRa Frog)

Use

Xenopus expression

Expression

Mammalian

Mutation

lacks the putative death domain (contains amino a…

Promoter

CMV

Available Since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-hs-3xHA-deltaBBox-LIN-41

Plasmid

#52719

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the BBox domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 194-320

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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