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pC0.018

Plasmid

#119622

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

dCas9+deg-tag

Use

Synthetic Biology

Mutation

bp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.006

Plasmid

#119670

Purpose

Level 0 part. Promoter, cpc560 promoter, extended spacer region, upstream TFB site region duplicated.

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Pcpc560-Ux2

Use

Synthetic Biology

Mutation

bp 89 T to C, bp 187 C to A, Duplicated upstream …

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1-neoR

Plasmid

#117048

Purpose

In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

Depositor

Michael Jewett

Publication

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

neoR

Expression

Bacterial

Promoter

T7

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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BSP609

Plasmid

#122254

Purpose

expression vector for mTFP under EFT3 promoter

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mTFP

Expression

Worm

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BPK2013-BsCas12b

Plasmid

#121952

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Publication

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

BsCas12b

Tags

3xHA tag and 10xHis tag

Expression

Bacterial

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0029

Plasmid

#117541

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

crRNA (LbCas12a)

Expression

Plant

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0028

Plasmid

#117540

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

crRNA (AsCas12a)

Expression

Plant

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCDB325

Plasmid

#113669

Purpose

expresses Ulp1_R1 in Escherichia coli

Depositor

Christopher Bahl

Publication

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R1

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAGM16841

Plasmid

#105416

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

STOP codon and nos terminator

Use

Synthetic Biology

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1355

Plasmid

#108994

Purpose

generic knockin vector for C-terminal tagging with GFP

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

GFP

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTC366

Plasmid

#91215

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, ribozyme processing)

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Expression

Plant

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0808

Plasmid

#91024

Purpose

Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

EC1.2

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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POT5-TDH3p-ycrtYB-ADH1t

Plasmid

#65358

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3p-ycrtYB-ADH1t

Use

Unspecified

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-T-TEF2

Plasmid

#65340

Purpose

construction of TEF2 terminator in HCKan-P receiving vector

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2 terminator

Use

Synthetic Biology

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-P-TDH3

Plasmid

#65336

Purpose

construction of TDH3 promoter in HCKan-P receiving vector

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3 promoter

Use

Synthetic Biology

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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POT2-TDH3p-ycrtE-ADH1t

Plasmid

#65352

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3p-ycrtE-ADH1t

Use

Unspecified

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-T-ADH1

Plasmid

#65339

Purpose

construction of ADH1 terminator in HCKan-P receiving vector

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ADH1 terminator

Use

Synthetic Biology

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-P-CYC1

Plasmid

#65338

Purpose

construction of CYC1 promoter in HCKan-P receiving vector

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1 promoter

Use

Synthetic Biology

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pJK_proB_amiE

Plasmid

#59836

Purpose

Constitutively expresses AmiE with proB promoter

Depositor

Timothy Whitehead

Publication

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Tags

6x-His

Expression

Bacterial

Promoter

proB

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK1_amiE

Plasmid

#59843

Purpose

Constitutively expresses AmiE with proK1 promoter

Depositor

Timothy Whitehead

Publication

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Tags

6x-His

Expression

Bacterial

Promoter

proK1

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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