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C1ORF43(1-41)-3xFLAG

Plasmid

#130881

Purpose

Expresses first 41 amino acids of human C1ORF43 with C-terminal 3xFLAG tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

C1ORF43 (C1orf43 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acids 44-253

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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deltaPYD-NLRP11.MYC

Plasmid

#131197

Purpose

mammalian expression

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

deltaPYD-NLRP11 (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

deletion of the pyrin domain (amino acids 1-124 m…

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B2 G120

Plasmid

#123077

Purpose

Gateway entry clone encoding human MAP1LC3B2 G120

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Use

Gateway Cloning

Mutation

Deleted amino acids 121-125. Stop codon after G12…

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSBbi-Puro_LPAR_AKAR-mut

Plasmid

#125207

Purpose

SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter

Depositor

Eric Davis

Insert

LPAR_AKAR-mut

Use

Transposon

Tags

EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

Expression

Mammalian

Mutation

FoxO1 Thr-24 changed to Val rendering this inacti…

Promoter

EF1a/RPBSA

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-I766FV111FL114F-HA-GFP

Plasmid

#120909

Purpose

Expresses Ptch1-B (I766FV111FL114F)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pNR2F1.1.0-gDNA

Plasmid

#112484

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR2F1

Depositor

Kevin White

Insert

NR2F1 (NR2F1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pZNF416-donor

Plasmid

#112372

Purpose

CRISPR donor plasmid to tag human transcription factor ZNF416 with GFP

Depositor

Kevin White

Insert

ZNF416 homology arms flanking EGFP-IRES-Neo cassette (ZNF416 Human)

Use

CRISPR

Mutation

hg38:19:57572882:A>G,hg38:19:57572932:C>G,h…

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Myc-gamma2S/deltaIL

Plasmid

#119730

Purpose

GABAA receptor expression (chimeric rat gamma2 short subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat delta subunit) Myc-tag near N-terminus

Depositor

Angel de Blas

Article

Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

Insert

GABRG2/GABRD (Gabrg2, Gabrd Rat)

Tags

cMyc epitope EQKLISEEDL inserted between fourth a…

Expression

Mammalian

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-P2A-GFP-PGK-Puro

Plasmid

#110863

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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PARP14 (PARP14A-c013)

Plasmid

#110254

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PARP14 (PARP14 Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7 - lacO

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3FLAG-MYB-TAD(251-327)

Plasmid

#105611

Purpose

transient express MYB TAD fragment with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAD fragment of MYB (mouse), amino acids 251-327 (Myb Mouse)

Tags

3*FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV2-OCT-HA-eGFP

Plasmid

#67481

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Expression

Mammalian

Promoter

attenuated CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARF1

Plasmid

#67453

Purpose

Mammalian expression of hArf1 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARF4

Plasmid

#67455

Purpose

Mammalian expression of hArf4 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4C-V5-His6

Plasmid

#67423

Purpose

Expression of hArl4c with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARL4-Myc

Plasmid

#67356

Purpose

Mammalian expression of hArl4 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL11

Plasmid

#67340

Purpose

hArl11 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 261 G to A; silent mutation; bp 442 T to C; aa…

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL11-no stop

Plasmid

#67341

Purpose

hArl11 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 442 T to C; aa C148R

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARFRP1-no stop

Plasmid

#67343

Purpose

hArfrp1 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARFRP1 (ARFRP1 Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-SARA-no stop

Plasmid

#67347

Purpose

hSara without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only