We narrowed to 118,874 results for: mpi

1. lentiCRISPR v2-Blast

   Plasmid

   #83480

   Purpose

   Mammalian expression of Cas9 and sgRNA scaffold

   Depositor

   Mohan Babu

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   EF-1α

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a-BirA

   Plasmid

   #20857

   Depositor

   Alice Ting

   Article

   Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

   Insert

   BirA
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTNS2

   Plasmid

   #64968

   Purpose

   Tn7 transposase expression

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   tnsABCD
   

   Expression

   Bacterial

   Available Since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCBASceI

   Plasmid

   #26477

   Purpose

   I-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI site

   Depositor

   Maria Jasin

   Article

   Richardson et al Genes Dev. 1998 Dec 15. 12(24):3831-42.

   Insert

   pCBASceI
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHyo245

   Plasmid

   #173147

   Purpose

   Expresses pheS_A294G by dual T7 promoter in e.coli

   Depositor

   Seokhee Kim

   Article

   Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

   Insert

   pheS_A294G
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHelper_TS_V2_ampR

   Plasmid

   #214467

   Purpose

   Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.

   Depositor

   Scott Saunders

   Article

   Saunders et al bioRxiv 2023

   Insert

   CspRecT / Bxb-1
   

   Use

   Synthetic Biology

   Promoter

   XylS / AraC

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

   Plasmid

   #175574

   Purpose

   Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD-iPASnFR-L

   Plasmid

   #225238

   Purpose

   Bacterial expression of iPASnFR-L

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-L
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-iPASnFR-H

   Plasmid

   #225237

   Purpose

   Bacterial expression of iPASnFR-H

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-H
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 HA 14-3-3 sigma

    Plasmid

    #11946

    Depositor

    Michael Yaffe

    Article

    Wilker et al J Biol Chem. 2005 May 13. 280(19):18891-

    Insert

    Stratifin (SFN Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKD4

    Plasmid

    #45605

    Depositor

    Barry L. Wanner

    Article

    Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

    Insert

    FRT-kan-FRT
    

    Expression

    Bacterial

    Promoter

    R6K

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. JC Mad-1

    Plasmid

    #25626

    Depositor

    Peter Howley

    Article

    Howley et al J Virol. 1980 Dec . 36(3):878-82.

    Insert

    JC Virus Mad-1
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

13. tol2-mpx-mCherry

    Plasmid

    #29585

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

    Insert

    mpx-mCherry
    

    Use

    Zebrafish expression

    Available Since

    June 3, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pXJ-Myc-IRF8

    Plasmid

    #232668

    Purpose

    transient expresses IRF8 in mammalian cells

    Depositor

    Chen-Yang Shen

    Insert

    IRF8 (IRF8 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICH47751

    Plasmid

    #48002

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. CMV-LgBit

    Plasmid

    #234738

    Purpose

    Mammalian expression of large NanoBit fragment (LgBit)

    Depositor

    Nevin Lambert

    Article

    Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

    Insert

    LgBit
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

    Plasmid

    #105648

    Purpose

    Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks

    Depositor

    Sandra Rieger

    Insert

    Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)
    

    Expression

    Mammalian

    Available Since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMPRA1

    Plasmid

    #49349

    Purpose

    Basic backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) libraries

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMT416

    Plasmid

    #8607

    Depositor

    Robert Hartley

    Article

    Hartley et al J Mol Biol 1988 Aug 20;202(4):913-5.

    Insert

    barnase, barstar
    

    Tags

    phoA signal sequence to barnase

    Expression

    Bacterial

    Available Since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CMV-FLEX-SaCas9-U6-sgVamp2

    Plasmid

    #159920

    Purpose

    Mutagenesis of Vamp2

    Depositor

    Larry Zweifel

    Insert

    Vamp2 (Vamp2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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