We narrowed to 66,466 results for: ELL
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Plasmid#110486PurposeLentiviral vector to express wild-type N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged wild type huntingtin exon1 fragment with 23 repeats of Q)DepositorInsertHomo sapiens huntingtin (HTT Human)
UseLentiviralTagsMycExpressionMammalianMutationShort 3'UTR wild type huntingtin exon1 fragm…PromoterUbCAvailable SinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
ExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailable SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK280 (mCherry2-NeoR)
Plasmid#72796PurposeC-terminal tagging with mCherry2 using CRIPSR/Cas9DepositorInsertmCherry2-Bsr
UseCRISPRTagsmCherry2ExpressionMammalianAvailable SinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF[TAG]
Plasmid#164579PurposeMachinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codonsDepositorInsertsMj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]
ExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoteraraBAD, glnS, and proKAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-BSD
Plasmid#174819PurposeMammalian expression of SpCas9 prime editor 2 with P2A-BSD markerDepositorInsertPE2-P2A-BSD
TagsP2A-BSD and SV40 bpNLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_sgRNA_hygro-tagBFP-lox5171
Plasmid#162070Purpose3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistanceDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralExpressionMammalianPromoterU6, EF1aAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-mCherry-GPI
Plasmid#65299Purposesynchronize trafficking of mCherry-GPI from the ER (RUSH system)DepositorInsertGPI anchor
UseLentiviralTagsIL-2 Signal Sequence and Streptavidin Binding Pro…PromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVTU_260_BirA
Plasmid#63682PurposeEncodes E. coli biotin ligase gene (BirA) for expression in yeast cellsDepositorInsertBirA
Tags6X Histidine and TEV cleavage siteExpressionYeastPromoterADH1/yeastAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
CaTCH_empty-backbone
Plasmid#157746PurposeLentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequencesDepositorTypeEmpty backboneUseLentiviral; Catch barcode cloningExpressionMammalianAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-ShadowR
Plasmid#132668PurposeExpresses ShadowR in mammalian cellsDepositorInsertShadowR
ExpressionMammalianPromoterCMVAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-EF1A>Tet3G:IRES:Neo
Plasmid#198756PurposeVector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418DepositorInsertTet3G, IRES, Neo
UseLentiviralPromoterEF1AAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HIFR
Plasmid#192946PurposeLentiviral production of HIF-1alpha reporterDepositorInsertHIF-1alpha reporter
UseLentiviralPromotermCMVAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Neo-TRE-CMV-Cre-rtTA
Plasmid#165457PurposeTetracycline or doxycycline inducible CRE expression from AAVS1 locusDepositorInsertCRE
ExpressionMammalianPromoterTight TRE promoterAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
HyperADAR control
Plasmid#166969PurposeExpress the Hyperactive catalytic domain of Drosophila ADAR and linker at the 5' end of this domain, in the MSCV-IRES-GFPDepositorInsertlinker-hyperADAR
UseRetroviralTagslinker-HyperADARExpressionMammalianPromoterMSCVAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
ORF3a-EGFP
Plasmid#165121Purposemammalian expression and localizationDepositorAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Palmbow
Plasmid#158993PurposeMulticolor membrane-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing palmitoylated mCherry, mEYFP or mCerulean upon Cre recombination)DepositorInsertPalmbow
TagsThe default expressed EBFP2 is fused to H2B; oth…ExpressionMammalianPromoterCAGAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Lyn11-FRB
Plasmid#155228Purposemembrane anchor for FKBP-FRB systemDepositorInsertMembrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system
ExpressionMammalianAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-mVenus-Twin-Strep
Plasmid#113891PurposeMammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusionDepositorTypeEmpty backboneUseLentiviralTags3C-mVenus-Twin-StrepPromoterCMV-MIE-TetO2Available SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-20X-UAS-ADARcd
Plasmid#81173Purposeidentification of cell-specific targets of RNA binding proteinsDepositorInsertUAS-ADARcd
ExpressionInsectPromoterUASAvailable SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-CMV-CC3AI
Plasmid#78909PurposeIndicator of caspase-3-like protease activityDepositorInsertCC3AI
UseLentiviralTagsHA and MycMutationS320A, deletion of AA1-200PromoterCMVAvailable SinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only