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tagRFP-GPHN.FingR-HaloTag

Plasmid

#236112

Purpose

Half of chemically inducible GFE3. When co-expressed with 236113 can degrade inhibitory synapses in presence of TH. (trimethoprim-HaloTag ligand).

Depositor

Don Arnold

Article

Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

Insert

tagRFP-GPHN.FingR-HaloTag

Expression

Mammalian

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5415

Plasmid

#229030

Purpose

Expression vector for 10xHis-phi29 DNA polymerase (wild-type): PT7-lacO-g10leader-p2 wt-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP_wt

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5444

Plasmid

#231064

Purpose

Expression vector for 10x-phi29 DNA polymerase (PovSak): PT7-lacO-g10leader-10xHis-p2 povsak-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP_PovSak

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5448

Plasmid

#231065

Purpose

Expression vector for 10x-phi29 DNA polymerase (PovSakOk): PT7-lacO-g10leader-10xHis-p2 povsakok-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP_PovSakOk

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5452

Plasmid

#231067

Purpose

Inactive tetO-10xHis-phi29 DNA polymerase wild-type reporter: PT7-tetO-B0035-10xHis-p2 dead-T7 terminator, AmpR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-tetO-B0035-10xHis-phi29 DNAP dead-T7term

Expression

Bacterial

Promoter

PT7-tetO

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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POC1554

Plasmid

#238471

Purpose

pMOBC360_VR: Vector Right (VR) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Lck-mCerulean3

Plasmid

#234538

Purpose

to label the membrane of cells with a fluorescent protein (mCerulean3)

Depositor

Hajime Hirase

Article

Wang et al Anat Sci Int. 2025 Jun 27. doi: 10.1007/

Insert

Lck-mCerulean3

Use

AAV

Mutation

None

Promoter

Short CAG

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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POC1525

Plasmid

#226496

Purpose

pMOBC360_VM: Vector Middle (VM) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1520

Plasmid

#221581

Purpose

pMOBK360_VR: Vector Right (VR) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1518

Plasmid

#221579

Purpose

pMOBK360_VL: Vector Left (VL) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1519

Plasmid

#221580

Purpose

pMOBK360_VM: Vector Middle (VM) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IARS2

Plasmid

#232337

Purpose

Expresses human IARS2 (ENST00000366922.3, NM_018060.4) in mammalian cells

Depositor

Quanfu Ma

Insert

IARS2 (IARS2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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P-fusA2

Plasmid

#235656

Purpose

Reports Cur activity in Bacteroides thetaiotaomicron

Depositor

Guy Townsend

Article

Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.

Insert

Bacteroides thetaiotaomicron fusA2 promoter

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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DriD_78-327

Plasmid

#237853

Purpose

DriD missing the wHTH DNABD used to crystallize the apo protein

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD 78-327

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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Hv_HPT:pCSRM2C:Gus_introns

Plasmid

#215191

Purpose

Evaluating guard cell specific promotors in Barley

Depositor

Penny Hundleby

Article

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

Scream2

Promoter

BdSCRM2

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pUASTattB-cytoFLARE1.0-TEV

Plasmid

#234519

Purpose

Transcriptional reporter for detecting calcium transients in Drosophila larvae (TEVp)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

CaM-V5-TEVp

Expression

Insect

Available Since

May 1, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-hPLEKHA7-Myc

Plasmid

#236538

Purpose

For expression of PLEKHA7 (human) in mammalian cells

Depositor

Sandra Citi

Article

Paschoud et al Tissue Barriers. 2014 Jan 1;2(1):e28755.

Insert

PLEKHA7 (PLEKHA7 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-ALFANb-HaloTag-GB1-dWPRE

Plasmid

#231600

Purpose

Express ALFANb-HaloTag

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

ALFANb-HaloTag

Use

Lentiviral

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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TLCV2 - sgAP2M1

Plasmid

#202758

Purpose

Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2M1

Depositor

Christopher Counter

Article

Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

Insert

gRNA targeting AP2M1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-G-iRFP720

Plasmid

#229177

Purpose

lvl 0 5 gene

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

iRFP720

Use

Synthetic Biology

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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