Addgene

We narrowed to 10,181 results for: AGA

Showing: 1021 - 1040 of 10181 results

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Kohinoor 2.0/pcDNA3

Plasmid

#182315

Purpose

Positively reversibly photoswitchable fluorescent protein

Depositor

Takeharu Nagai

Article

Wazawa et al Microscopy (Oxf). 2021 Aug 9;70(4):340-3

Insert

Kohinoor 2.0

Use

Tags

No tag

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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RS314-SN

Plasmid

#17539

Depositor

Bert Vogelstein

Article

Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

Insert

Gal1-p53 (TP53 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
GeNL(Ca2+)_480/pcDNA3

Plasmid

#85205

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_480

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pQIq_MRGS_HIS8_(27G2)_FLAG

Plasmid

#199616

Purpose

Encodes DARPin-FLAG 27G2 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG 27G2

Use

Tags

8xHis and FLAG

Expression

Bacterial

Mutation

Promoter

T5

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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Lyn-OeNL_pcDNA3

Plasmid

#89528

Purpose

Orange color variant of bright luminescent protein, targeted to the plasma membrane

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

Lyn

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-AksgRNA

Plasmid

#121955

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b single chimeric gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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tubulin-ReNL_pcDNA3

Plasmid

#89530

Purpose

Red color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

b-tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Y-RAN-RFP6

Plasmid

#106424

Purpose

Y-RAN-RFP6 (RANbody RFP6::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-RFP6

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-Q

Plasmid

#64980

Purpose

Expresses the E108Q mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Use

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E108Q

Promoter

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR SD Age HsSAS-6

Plasmid

#46381

Purpose

For expression of C-terminal fusions.

Depositor

Pierre Gonczy

Article

Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

Insert

SAS-6 (SASS6 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGK401red-rtetTAK8N, L131L

Plasmid

#165968

Purpose

Expresses rTetTA (K8N, L131L) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

tetR

Use

Synthetic Biology

Tags

three copies of VP16 (VP48)

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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NFkBm2

Plasmid

#15090

Depositor

David Baltimore

Article

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

miR-146a promoter

Use

Tags

Expression

Mammalian

Mutation

2 NFkB binding sites mutated

Promoter

Available since

June 6, 2007

Availability

Academic Institutions and Nonprofits only
pUC19-U6-AasgRNA

Plasmid

#121954

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b single chimeric gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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GeNL(Ca2+)_250/pcDNA3

Plasmid

#85206

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_250

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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Kohinoor/pcDNA3

Plasmid

#67771

Purpose

Kohinoor for mammalian cell expression in cytosol

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only
CeNL-H2B_pcDNA3

Plasmid

#89511

Purpose

Cyan color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Use

Tags

histone 2B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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PcDNA3.1-2Strep

Plasmid

#210343

Purpose

Empty Vector for molecular cloning

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

2Strep tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGPΩTp

Plasmid

#130660

Purpose

Insertional mutagenesis of GP transposon into chromosome of B. cenocepacia. Trimethoprim selection for plasmid.

Depositor

Miguel Valvano

Article

Flannagan et al Infect Immun. 2007 Apr;75(4):1679-89. Ep

Insert

Ω trimethoprim cassette

Use

Tags

Expression

Bacterial

Mutation

Addition of Ω sites next to trimethoprim cassette…

Promoter

dhfr promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Actin/pcDNA3

Plasmid

#67776

Purpose

Actin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

Actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pQIq_MRGS_HIS8_( E3_5)_FLAG

Plasmid

#199612

Purpose

Encodes DARPin-FLAG E3_5 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG E3_5 (control)

Use

Tags

8xHis and FLAG

Expression

Bacterial

Mutation

Promoter

T5

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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