Addgene

We narrowed to 6,939 results for: Cad

Showing: 1021 - 1040 of 6939 results

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pKJ247

Plasmid

#228714

Purpose

Mammalian expression of U6 epPsaCas12f RNF2 targeting guide

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Science 0, eadr6006

Insert

u6-RNF2 targeting guide

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAM254

Plasmid

#226898

Purpose

mEOS3.2-IDR*-(7aaSG)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Use

Tags

7aaSG Prescission GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTrc-CysGA-Im7 WT

Plasmid

#201081

Purpose

Im7 WT inserted into CysGA at G364 via GS linker

Depositor

Shu Quan

Article

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Im7

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTrc

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTrc-CysGA-Linker only

Plasmid

#201080

Purpose

GS linker inserted into CysGA at G364

Depositor

Shu Quan

Article

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Linker only

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTrc

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19_BAS1509

Plasmid

#82354

Purpose

T7 in vitro transcription template for Bas mRNA

Depositor

Nils Walter

Article

Chatterjee et al Proc Natl Acad Sci U S A. 2021 Apr 20;11

Insert

BAS1509 hypothetical protein

Use

Unspecified

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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AlACR2_EYFP_pcDNA3.1

Plasmid

#161023

Purpose

Expresses AlACR2 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR2

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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AlACR3_EYFP_pcDNA3.1

Plasmid

#161024

Purpose

Expresses AlACR3 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR3

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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GtCCR1_EYFP_pcDNA3.1

Plasmid

#159120

Purpose

This plasmid encodes GtCCR1 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

Insert

GtCCR1

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKEW395_AcrIIA14_truncated

Plasmid

#138284

Purpose

Expression of truncated AcrIIA14 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

truncated AcrIIA14

Use

CRISPR

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lenti

Plasmid

#138312

Purpose

Lenti expression of AcrIIA14, C-terminus

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA14, C terminus

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKEW353_AcrIIA13_truncated

Plasmid

#138282

Purpose

Expression of truncated AcrIIA13 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

truncated AcrIIA13

Use

CRISPR

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKEW398_AcrIIA15_truncated

Plasmid

#138286

Purpose

Expression of truncated AcrIIA15 protein

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA15

Use

CRISPR

Tags

SFGFP

Expression

Bacterial

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pNOC-FLUC

Plasmid

#98140

Purpose

Gateway compatible construct for N' terminal fusion or promoter driven firefly luciferase reporter, hygromycin resistance cassette

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Tags

HA

Expression

Mutation

Promoter

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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P0050::kaiBC

Plasmid

#101828

Purpose

drives expression of kaiBC from an rpaA-independent promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kanamycin casette and p0050 promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::glgP_gap1

Plasmid

#101845

Purpose

Replaces native promoter of glgP_gap1 with Ptrc promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

CmR casette followed by ptrc promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::zwf_fbp_opcA

Plasmid

#101846

Purpose

Replaces native promoter of zwf_fbp_opcA with Ptrc promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

sp/st resistance casette followed by ptrc promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Ptrc::gnd

Plasmid

#101844

Purpose

drives expression of gnd using Ptrc promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kan casette followed by ptrc promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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zwf-fbp-opcA-

Plasmid

#101839

Purpose

deletes zwf_fbp_opcA locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Kanamycin casette (in place of synpcc7942_2333-2335 operon)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pJexpress414_OmpA171_H3478

Plasmid

#64292

Purpose

OmpA redesign hybrid with wild-type strands 3478

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H3478

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H346

Plasmid

#64312

Purpose

OmpA redesign hybrid with wild-type strands 346

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H346

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only